Main Article Content
Abstract
In present study, the presence of bioactive compounds in A. mexicana flowers were analyzed and also evaluated the in vitro antioxidant properties of A. mexicana flowers. The flower extract showed the presence of alkaloids, flavonoids, saponins, tannins, phytosterol, triterpenoids, glycosides, anthraquinones and phenols. The total phenolic and flavonoid content were found to be 25.40 ± 1.20 μg gallic acid equivalents and 14.30 ± 0.20 μg quercetin equivalents, respectively. The carbohydrate and protein content was found to be 4.10 ± 0.24 mg/g and 2.10 ± 0.30 mg/g of the flower extract respectively. HPLC analysis of A. mexicana flower extract revealed the presence of biologically active components such as gallic acid, rutin, caffeic acid, quercetin and ferulic acid. A. mexicana flower extract exhibited 81.33% inhibition in DPPH assay, 85% in ABTS radical assay, 86% superoxide scavenging, 76% NO scavenging, 75% hydroxyl radical scavenging, 77% radicals indicating hydrogen peroxide radical scavenging potential of the flower extract. The antioxidant activity observed in the ethanolic extarct of A. mexicana flower may be related to the presence of significant amounts of total phenolics and flavonoids. Hence, A. mexicana flower extract could serve as natural sources of antioxidants and could be used in the treatment of free radical mediated diseases.
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References
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References
I.A. Siddiqui, S.S. Shaukat, G.H. Khan and M.J. Zaki, Evaluation of Argemone mexicana for Control of Root-Infecting Fungi in Tomato, J. Phytopathol., 150, 321 (2002); https://doi.org/10.1046/j.1439-0434.2002.00762.x
I. Bhattacharjee, S.K. Chatterjee, S. Chatterjee and G. Chandra, Antibacterial Potentiality of Argemone mexicana Solvent Extracts against Some Pathogenic Bacteria, J. Mem. Inst. Oswaldo Cruz., 101, 645 (2006); https://doi.org/10.1590/S0074-02762006000600011
W. Rahman and M. Ilyas,Flower Pigments. Flavonoids from Argemone mexicana linn. (Papaveraceae), J. Org. Chem., 27, 153 (1962); https://doi.org/10.1021/jo01048a037
R.D. Bhalke, Y.P. Mandole and N.B. Mali, Phytochemical Investigation and Effect of Various Extracts of Argemone mexicana (Papaveraceae) Leaves Onclonidine and Haloperidol-Induced Catalepsy in Mice, J. Pharm. Res., 2, 765 (2009).
G. Ji, S.K. Shukla, P. Dwivedi, S. Sundaram and R. Prakash, Inhibitive Effect of Argemone mexicana Plant Extract on Acid Corrosion of Mild Steel, Ind. Eng. Chem. Res., 50, 11954 (2011); https://doi.org/10.1021/ie201450d
J.B. Harborne, Phytochemical Methods, Chapman & Hall Int.: New York, Ed. 3 (1998).
C.K. Kokate, Pharmacognosy. Nirali Prakasham: Mumbai, India, Ed. 16 (2001).
V.L. Singleton, R. Orthofer and R.M. Lamuela-Raventos, Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-ciocalteu Reagent, Methods Enzymol., 299, 152 (1999); https://doi.org/10.1016/S0076-6879(99)99017-1
S. Kumazawa, M. Taniguchi, Y. Suzuki, M.K. Shimura, M.S. Kwon and T. Nakayama, Antioxidant Activity of Polyphenols in Carob Pods, J. Agric. Food Chem., 50, 373 (2002); https://doi.org/10.1021/jf010938r
C. Quettier-Deleu, B. Gressier, J. Vasseur, T. Dine, C. Brunet, M. Luyckx, M. Cazin, J.-C. Cazin, F. Bailleul and F. Trotin, Phenolic Compounds and Antioxidant Activities of Buckwheat (Fagopyrum esculentum Moench) Hulls and Flour, J. Ethnopharmacol., 72, 35 (2000); https://doi.org/10.1016/S0378-8741(00)00196-3
W. Brand-Williams, M.E. Cuvelier and C. Berset, Use of a Free Radical Method to Evaluate Antioxidant Activity, LWT-Food Sci. Technol., 28, 25 (1995); https://doi.org/10.1016/S0023-6438(95)80008-5
R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang and C. Rice-Evans, Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay, Free Radic. Biol. Med., 26, 1231 (1999); https://doi.org/10.1016/S0891-5849(98)00315-3
N. Smirnoff and Q.J. Cumbes, Hydroxyl Radical Scavenging Activity of Compatible Solutes, Phytochemistry, 28, 1057 (1989); https://doi.org/10.1016/0031-9422(89)80182-7
R.J. Ruch, S.J. Cheng and J.E. Klaunig, Prevention of Cytotoxicity and Inhibition of Intercellular Communication by Antioxidant Catechins Isolated from Chinese Green Tea, Carcinogenesis, 10, 1003 (1989); https://doi.org/10.1093/carcin/10.6.1003
L. Marcocci, J.J. Maguire, M.T. Droylefaix and L. Packer, The Nitric Oxide-Scavenging Properties of Ginkgo Biloba Extract EGb 761, Biochem. Biophys. Res. Commun., 201, 748 (1994); https://doi.org/10.1006/bbrc.1994.1764
M. Fontana, L. Mosca and M.A. Rosei, Interaction of Enkephalins with Oxyradicals, Biochem. Pharmacol., 61, 1253 (2001); https://doi.org/10.1016/S0006-2952(01)00565-2
N. Joshi, S. Bhatt, S. Dhyani and J. Nain, Phytochemical Screening of Secondary Metabolites of Argemone mexicana Linn. Flowers, Int. J. Curr. Pharm. Res., 5, 144 (2013).
M. Goswami, A. Yadav, R. Bhardwaj and Y.C. Joshi, Antioxidant Properties of Methanolic Extracts of Argemone mexicana, Res. J. Med. Plant, 8, 167 (2014); https://doi.org/10.17311/rjmp.2014.167.177
I. Gulcin, Z. Huyut, M. Elmastas and H.Y. Aboul-Enein, Radical Scavenging and Antioxidant Activity of Tannic Acid, Arab. J. Chem., 3, 43 (2010); https://doi.org/10.1016/j.arabjc.2009.12.008