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Abstract

The proposed oligosaccharide mixture of Donkey’s milk has shown significant stimulation of antibody, delayed type hypersensitivity response to sheep red blood cells in BALB/c mice. The orally treated animals showed a six time increase in haemagglutinating antibody (HA) titre, two times increase in haemolytic plaque-forming cells (PFC) and delayed type hyper-sensitivity (DTH) response. The nonspecific immune response of treated animals also showed a two times increase in macrophage migration index (MMI). Two novel oligosaccharides have been isolated from this oligosaccharide mixture obtained from donkey’s milk having immunostimulant activity. These compounds were isolated by a combination of gel filtration chromatography, silica gel column chromatography of derivatized oligosaccharides while their homogeneity was confirmed by HPLC. The structures of these isolated oligosaccharides were elucidated on the basis of NMR spectroscopy, mass spectrometry and also with help of Structure reporter groups.

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Oligosaccharide Immunostimulant Donkey milk Equinose Asinose

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K. Ranjan, A., S. Rathore, R., Deepak, D., Khare, A., Sahai, R., & Srivastava, V. (2016). Immunostimulant Fractions of Novel Hexa and Heptasaccharide from Donkey’s Milk. Asian Journal of Organic & Medicinal Chemistry, 1(2), 55–60. https://doi.org/10.14233/ajomc.2016.AJOMC-P17
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