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Vol. 31 No. 2 (2019): Vol. 31 No. 2

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Quantitation of Vitamin E in Pharmaceutical Formulations and Oil Samples Using Spectrophotometric Method

https://doi.org/10.14233/ajchem.2019.21554
Ashwani Kumar
Department of Chemistry, Kurukshetra University, Kurukshetra-136119, India
Mamta Kamboj
Department of Chemistry, Kurukshetra University, Kurukshetra-136119, India

Corresponding Author(s) : Ashwani Kumar

akumarchem@kuk.ac.in

Asian Journal of Chemistry, Vol. 31 No. 2 (2019): Vol. 31 No. 2

Abstract

A novel, rapid and facile procedure is described for the determination of vitamin E. It involves the reduction of Fe(III) to Fe(II) with vitamin E that results the formation of pink colored complex of reduced Fe(II) with dimethyl glyoxime in presence of pyridine in pH range between 6.5-7.2. The absorbance of the complex was measured at 512 nm. Beer′s law was obeyed in the range 2.0 to 5.2 μg/mL of vitamin E with molar absorptivity of 1.23 × 104 L mol-1 cm-1 and the Sandell′s sensitivity was 0.0351 μg/cm2. The coefficient of determination was r2 = 0.997 with the relative standard deviation 1.1 %. The various factors which affect the resulting complex were optimized to obtain maximum absorption. The proposed method is satisfactorily utilized for pure as well as various dosage forms of vitamin E in pharmaceutical formulations which contains common reductants by pre-treatment of the samples.

Keywords

Spectrophotometer Vitamin E Ferric chloride Dimethyl glyoxime
Kumar, A., & Kamboj, M. (2018). Quantitation of Vitamin E in Pharmaceutical Formulations and Oil Samples Using Spectrophotometric Method. Asian Journal of Chemistry, 31(2), 279–282. https://doi.org/10.14233/ajchem.2019.21554
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