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Vol. 32 No. 6 (2020): Vol 32 Issue 6

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Spectrophotometric and RP-HPLC Methods for Determining Cefotaxime Sodium in Pharmaceutical Preparations

https://doi.org/10.14233/ajchem.2020.22524
Lamya A. Sarsam
Department of New and Renewable Energy, College of Science, Mosul University, Mosul, Iraq
Salim A. Mohammed
Department of Chemistry, College of Science, Mosul University, Mosul, Iraq
Sahar A. Fathe
General Company for Communications Equipment and Power Al-Kindy Factory, Mosul, Iraq

Corresponding Author(s) : Lamya A. Sarsam

luma_sarsam@yahoo.com; lamyasarsam@uomosul.edu.iq

Asian Journal of Chemistry, Vol. 32 No. 6 (2020): Vol 32 Issue 6

Abstract

A rapid, simple and sensitive spectrophotometric and RP-HPLC methods have been developed for the quantitative determination of cefotaxime-Na in both pure and dosage forms. The spectrophotometric method was based on diazotization of cefotaxime-Na and then coupling with 8-hydroxyquinoline in an alkaline medium. The resulting azo dye exhibited maximum absorption at 551 nm with a molar absorptivity of 0.597 × 104 L mol-1 cm-1. Beer′s law was obeyed over the range 10-700 μg/25 mL (i.e. 0.4-28.0 ppm) with an excellent determination coefficient (R2 = 0.9993). The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.0194 and 0.3765 μg mL-1, respectively. The recoveries were obtained in the range 97.3-102.5% and the relative standard deviation (RSD) was better than ± 1.56. The HPLC method has been developed for the determination of cefotaxime-Na. The analysis were carried out on a C18 column and a mobile phase composed of acetonitrile and phosphate buffer solution (0.024M KH2PO4 and 0.01M H3PO4) at pH 3.5 in the ratio of 60:40 (v:v), with a flow rate of 1.0 mL min-1 and UV detection at 258 nm. The proposed method showed good linearity (in a range of concentration 1.0-200 μg mL-1. The recovery percent and a relative standard deviations were found in the range 96 to 104.8% and ± 0.017 to ± 0.031%, respectively. Both methods were applied successfully to the assay of cefotaxime-Na in commercial injection preparations.

Keywords

Cefotaxime-Na RP-HPLC Spectrophotometry Diazometry
Sarsam, L. A., Mohammed, S. A., & Fathe, S. A. (2020). Spectrophotometric and RP-HPLC Methods for Determining Cefotaxime Sodium in Pharmaceutical Preparations. Asian Journal of Chemistry, 32(6), 1314–1320. https://doi.org/10.14233/ajchem.2020.22524
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