Issue

Vol. 30 No. 2 (2018): Vol 30 Issue 2

Copyright (c) 2018 AJC

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Extraction and Identification of Inulin-Type Fructo-Oligosaccharides from Dahlia pinnata L.

https://doi.org/10.14233/ajchem.2018.20965
Nur Kusmiyati
The Graduate School of Biotechnology, Universitas Gadjah Mada, Yogyakarta, Indonesia
Tutik Dwi Wahyuningsih
The Graduate School of Biotechnology, Universitas Gadjah Mada, Yogyakarta, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Gadjah Mada, Yogyakarta, Indonesia
Widodo
The Graduate School of Biotechnology, Universitas Gadjah Mada, Yogyakarta, Indonesia; Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia

Corresponding Author(s) : Widodo

widodohs@ugm.ac.id

Asian Journal of Chemistry, Vol. 30 No. 2 (2018): Vol 30 Issue 2

Abstract

A method for obtaining high yields of high-quality inulin from dahlia tubers was developed in the present study. The steps involved were the extraction of inulin, determination of inulin concentration and elucidation of inulin structure. The highest yield of inulin was obtained by ultrasonic- and microwave-assisted extraction (UMAE) (5.77 %), followed by stirring extraction (4.97 %) and indirect sonication (5.09 %). The concentrations of inulin extracted from dahlia tubers using UMAE were determined to be 41.94 % (w/w) and 33.52 % (w/w) by 3,5-dinitrosalicylic acid and high-performance liquid chromatography, respectively. Analysis of inulin extracted from dahlia tubers by Fourier-transform infrared spectroscopy confirmed the presence of an inulin-type fructan that is the same as commercial inulin. 1H NMR and 13C NMR spectroscopy indicated that it has a degree of polymerization of 19-20 and a molecular weight of 3.173-3.316 Da.

Keywords

Inulin Dahlia tubers Ultrasonic- and microwave-assisted extraction 3,5-Dinitrosalicylic acid HPLC NMR spectroscopy
Kusmiyati, N., Wahyuningsih, T. D., & Widodo, . (2017). Extraction and Identification of Inulin-Type Fructo-Oligosaccharides from Dahlia pinnata L. Asian Journal of Chemistry, 30(2), 355–358. https://doi.org/10.14233/ajchem.2018.20965
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. H.W. Modler, Int. Dairy J., 4, 383 (1994); https://doi.org/10.1016/0958-6946(94)90055-8.
  2. N.K. Kochnev and M.V. Kamenecheva, Jerusalem Artichoke-Bioenergy Culture of the XXI Century, Ares Publ, Moscow, p. 76 (2002) (In Russian).
  3. S. Widowati, T.C. Sunarti and A. Zaharani, Seminar Rutin Puslitbang Tanaman Pangan (2005).
  4. W. Lingyun, W. Jianhua, Z. Xiaodong, T. Da, Y. Yalin, C. Chenggang, F. Tianhua and Z. Fan, J. Food Eng., 79, 1087 (2007); https://doi.org/10.1016/j.jfoodeng.2006.03.028.
  5. Z. Lou, H. Wang, D. Wang and Y. Zhang, J. Carbohydr. Chem., 78, 666 (2009); https://doi.org/10.1016/j.carbpol.2009.05.029.
  6. M. Luisa, M.I. Nunes, F.M. Sequeira and A.E. Baptista, Alim. Nutr. Araraquara, 16, 221 (2005).
  7. S. Winarti, E. Harmayani, Y. Marsono and Y. Pranoto, J. Agritech., 33, 424 (2013).
  8. M. Hariono, M.F. Akbar, I. Sularsih, L. Najihah, S. Purwadi and A.W Nugrahani, Extraction, Identification and Acetylation of Inulin from dahlia tuber (Dahlia pinata cav.), The 9th National Symposium on Polymeric Materials 2009 (NSPM 2009), Residence Hotel, Uniten, Putrajaya, Indonesia, 14-16 December (2009).
  9. A. Pastore, S. Bernardini, L.D. Strologo, G. Rizzoni, C. Cortese and G. Federici, J. Chromatogr. A, 751, 187 (2001); https://doi.org/10.1016/S0378-4347(00)00444-8.
  10. G.L. Miller, Anal. Chem., 31, 426 (1959); https://doi.org/10.1021/ac60147a030.
  11. M. Dubois, K.A. Gilles, J.K. Hamilton, P.A. Rebers and F. Smith, Anal. Chem., 28, 350 (1956); https://doi.org/10.1021/ac60111a017.
  12. M. Grube, M. Bekers, D. Upite and E. Kaminska, Vib. Spectrosc., 28, 277 (2002); https://doi.org/10.1016/S0924-2031(02)00005-X.
  13. T. Barclay, M. Ginic-Markovic, P. Cooper and N. Petrovsky, J. Excipients Food Chem., 1, 27 (2010).
  14. D.M. Doddrell, D.T. Pegg and M.R. Bendall, J. Magn. Reson., 48, 323 (1969); https://doi.org/10.1016/0022-2364(82)90286-4.
  15. Y. Susdiana, Ekstraksi dan Karakterisasi Inulin dari Umbi Dahlia (Dahlia pinnata Cav). Skripsi, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Indonesia (1997) (Unpublished).
  16. S.J. Kays and S.F. Nottingham, Biology and Chemistry of Jerusalem artichoke: Helianthus tuberosus L., CRC Press, Boca Raton, FL. pp. 1-478 (2008).
  17. K. Vilkhu, R. Mawson, L. Simons and D. Bates, Innov. Food Sci. Emerg. Technol., 9, 161 (2008); https://doi.org/10.1016/j.ifset.2007.04.014.
  18. J. Silalahi and N. Hutagalung, Komponen-komponen bioaktif dalam makanan dan pengaruhnya terhadap kesehatan. Jurusan Farmasi. Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara, Medan, Indonesia (2010).
  19. S. Lee, L. Prosky and J.W. De Vries, J. AOAC Int., 75, 395 (1992).
  20. J. Matias, J. González, L. Royano and A.R. Barrena, J. Biomass Bioenergy, 35, 2006 (2011); https://doi.org/10.1016/j.biombioe.2011.01.056.
  21. X.Y. Wu and P.I. Lee, J. Appl. Polym. Sci., 77, 833 (2000); https://doi.org/10.1002/(SICI)1097-4628(20000725)77:4<833::AIDAPP17>3.0.CO;2-4.
  22. N. Petkova and P. Denev, Methods for Determination of Inulin. International Scientific-Practical Conference, University of Food Technologies, 24 July 2016.
  23. T. Barhatova, M. Nazarenko, M. Koguhova and I. Hripko, Foods Raw Mater., 3, 13 (2015); https://doi.org/10.12737/13115.
  24. Z. Yang, J. Hu and M. Zhao, Carbohydr. Polym., 83, 1997 (2011); https://doi.org/10.1016/j.carbpol.2010.11.006.
  25. S. Cerantola, N. Kervarec, R. Pichon, C. Magne, M.A. Bessieres and E. Deslandes, Carbohydr. Res., 339, 2445 (2004); https://doi.org/10.1016/j.carres.2004.07.020.
Read More
Author biographies is not available.
Download
PDF
Statistic
Read Counter : 1 Download : 0