Spectrophotometric Determination of Niacin Using 2,3-Dichloro-5,6-dicyano-1,4-benzoquinone

G.C. Nwanisobi; P.O. Ukoha

doi:10.14233/ajchem.2016.19888

Issue

Vol. 28 No. 11 (2016): Vol 28 Issue 11

Issue Published : August 10, 2016

This work is licensed under a Creative Commons Attribution 4.0 International License.

Spectrophotometric Determination of Niacin Using 2,3-Dichloro-5,6-dicyano-1,4-benzoquinone

https://doi.org/10.14233/ajchem.2016.19888

G.C. Nwanisobi

Department of Chemical Engineering, Faculty of Engineering and Technology, Madonna University (Akpugo Campus), Enugu State, Nigeria

P.O. Ukoha

Department of Pure and Industrial Chemistry, University of Nigeria, Nsukka, Nigeria

Corresponding Author(s) : G.C. Nwanisobi

glochinwa4real@yahoo.com

Asian Journal of Chemistry, Vol. 28 No. 11 (2016): Vol 28 Issue 11
Article Published : August 11, 2016

Abstract

A simple and sensitive spectrophotometric method is developed for the assay of niacin. The method is based on charge transfer complexation reaction of niacin as n-electron donor with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone as p-acceptor to form highly coloured charge transfer complex with 1:1 stoichiometry. The coloured product was quantified at 464 nm under the optimized experimental conditions. Beer’s law is obeyed over the concentration range of 5-130 μg/mL. The apparent molar absorptivity is calculated to be 1.02 × 10³ L mol^-1 cm^-1. Corresponding Sandell’s sensitivity is 1.85, detection and quantification limits are also reported. The proposed method is applied successfully to the determination niacin in pure and commercial forms with good average recovery of 97.82 % and low relative standard deviation of < 1. Statistical comparison of the results was performed using the student’s t-test and f-test at 95 % confidence level.

Keywords

Niacin Spectrophotometry Charge transfer complex ,3-Dichloro-5,6-dicyano-1,4-benzoquinone

Nwanisobi, G., & Ukoha, P. (2016). Spectrophotometric Determination of Niacin Using 2,3-Dichloro-5,6-dicyano-1,4-benzoquinone. Asian Journal of Chemistry, 28(11), 2371–2374. https://doi.org/10.14233/ajchem.2016.19888

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References

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References

R. Foster, Organic Charge–Transfer Complex, Academic Press, London (1967).

U. Ajali and B.K. Chukwurah, Bull. Chim. Farm., 140, 365 (2001).

U. Ajali, B. Ezema and P.O. Ukoha, J. Chem. Soc. Nigeria, 33, 23 (2008).

H.B. Hassib and Y.M. Issa, Egypt. J. Anal. Chem., 39, 329 (1996).

K. Nagaraju and K. Basavaiah, Acta Pol. Pharm., 69, 213 (2012).

C.C. Ezeanokette, K.G. Ngwoke, F.B. Okoye and P.A. Osadebe, Eur. Chem. Bull., 2, 1009 (2013).

H.N. Deepakumari and H.D. Revanasiddappa, J. Spectrosc., Article ID 671689 (2013); doi:10.1155/2013/671689.

H. Hala, E. Suad, A. Gadir, S. Refat and A. Adam, Int. J. Electrochem. Sci., 8, 5774 (2013).

E.Y. Frag, G.G. Mohamed, A.B. Farag and E.B. Yussof, Insight Pharm. Sci., 1, 47 (2011); doi:10.5567/IPHARMA-IK.2011.47.54.

I. Ramzin and E. Ehab, Int. J. Biomed. Sci., 8, 204 (2012).

F. Khalaf, J. Baghdad Foren. Sci., 10, 3 (2013).

E.A. Braude, R.P. Linstead and K.R. Wooldridge, J. Chem. Soc., 3070 (1956); doi:10.1039/jr9560003070.

V. Krishna and G. Sankar, E-J. Chem., 5, 493 (2008); doi:10.1155/2008/801545.

E. Khaled, Talanta, 75, 1167 (2008); doi:10.1016/j.talanta.2008.01.031.

D.S. Wishart, C. Knox, A.C. Guo, D. Cheng, S. Shrivastava, D. Tzur, B. Gautam and M. Hassanali, Nucl. Acids Res., 36(Suppl 1), D901 (2008); doi:10.1093/nar/gkm958.

The New Encyclopedia Britannica, vol. 8, edn 15, London (2012).

R.N. Nagi, R. Venkateshwara, R. Jayapal, D. Adukondalu and R. Karumakar, Int. J. Pharm. BioSci., 1, 479 (2011).

B. Sarangi, S. Chatterjee and K. Dutta, J. Anal. Chem., 68, 547 (1985).

P. Tiwari and P. Sathe, Adv. Biosci. Biotechnol., 1, 131 (2010); doi:10.4236/abb.2010.12018.

P.K. Zarzycki, P. Kowalski, J. Nowakowska and H. Lamparczyk, J. Chromatogr. A, 709, 203 (1995); doi:10.1016/0021-9673(95)00247-K.

Y. Hsieh and J. Chen, J. Rapid Commun. Mass Spectrom., 19, 3031 (2005); doi:10.1002/rcm.2171.

R. Sawant, R. Ahmed, S. Ramdin and S. Darade, Int. Res. J. Pharm., 3, 364 (2012).

A. Raza, Anal. Lett., 39, 2217 (2006); doi:10.1080/00032710600755322.

H. Bauer, G. Christian and J. Oreilly, Instrumental Analysis, Allyn and Bacon, Inc. Boston, pp. 178-179 (1978).

M. Refat, G. Mohammed and A. Fathi, Bulg. Chem. Commun., 45, 250 (2013).

International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use, ICH Harmonized of Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology, Complementary Guideline on Methodology, London (2005).

Issue

Vol. 28 No. 11 (2016): Vol 28 Issue 11

Spectrophotometric Determination of Niacin Using 2,3-Dichloro-5,6-dicyano-1,4-benzoquinone

Corresponding Author(s) : G.C. Nwanisobi

Abstract

Keywords

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