Simultaneous Determination of Paracetamol, Phenylephrine Hydrochloride and Loratadine

Abstract

A simple, selective, accurate RP-HPLC method was developed and validated for the analysis of paracetamol, phenylephrine hydrochloride and loratadine in commercially available tablet formulations. Chromatographic separation achieved isocratically using methanol:acetonitrile: water: THF (50:40:10:2.5 % v/v/v/v) as mobile phase at a flow rate of 0.8 mL/min. The retention time of phenylephrine, paracetamol and loratadine were found to be 2.563, 3.643, 5.779 min. respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation. It may be used for the estimation of these drugs in combined tablet dosage forms.