Determination of Natural Occurrence of Ochratoxin A in Naked Oats of China by HPLC
Yang Yang
Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, Department of Food Science, Beijing University of Agriculture, Beijing 102206, P.R. China
Hongxing Zhang
Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, Department of Food Science, Beijing University of Agriculture, Beijing 102206, P.R. China
Jingyu Gao
Beijing Entry-Exit Inspection and Quarantine Bureau of The Peoples Republic of China, Beijing 100621, P.R. China
Yuanhong Xie
Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, Department of Food Science, Beijing University of Agriculture, Beijing 102206, P.R. China
Xiaogong Wang
The National Research Center of Soybean Engineering and Technology, Northeast Agricultural University, Harbin 150030, P.R. China
Hui Liu
Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, Department of Food Science, Beijing University of Agriculture, Beijing 102206, P.R. China
Tao Han
Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, Department of Food Science, Beijing University of Agriculture, Beijing 102206, P.R. China
Corresponding Author(s) : Hongxing Zhang
hxzhang511@163.com
Asian Journal of Chemistry,
Vol. 24 No. 11 (2012): Vol 24 Issue 11
Ochratoxin A is a ubiquitous mycotoxin produced by different species of Aspergillus and Penicillium fungi. It is widespread in food and feed and its occurrence has been reported in cereals, cereal-derived products, dried fruits and spices. The aim of our study was to investigate the presence of ochratoxin A in naked oats which was widely consumed and commercialized in China. The analytical methods used in this study involved the extraction of ochratoxin A by acidified toluene, immunoaffinity (IAC) clean-up and high performance liquid chromatography (HPLC) quantification with fluorescence detection. The limit of detection for ochratoxin A was 0.5 μg kg-1. Levels and percentages of ochratoxin A contamination in different types of naked oats, 35 white oats, 56 upon dam naked oats and 48 Mongolia naked oats samples, were evaluated with incidences of 31, 23 and 33 %, respectively. The average of contamination by ochratoxin A found were 3.85, 2.33 and 2.38 μg kg-1, respectively, for white oats, upon dam naked oats and Mongolia naked oats. Experimental results showed that the contamination percentages and levels in 2010 were lower than usual norms (5 μg kg-1) established by the European commission in 2002.
Yang, Y., Zhang, H., Gao, J., Xie, Y., Wang, X., Liu, H., & Han, T. (2012). Determination of Natural Occurrence of Ochratoxin A in Naked Oats of China by HPLC. Asian Journal of Chemistry, 24(11), 5349–5351. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/9866
