Off-Line SPE-GC/MS Analysis of Lead Compounds Aromatherapy in Blood Plasma of Mice of Essential Oils Materials from Indonesian Aromatic Plants

Abstract

The aim of this study was to evaluate the analysis method for identify lead compounds aromatherapy in blood plasma by using off-line SPE-GC/MS. Identification and quantification of active compounds in the blood plasma were carried out with GC/MS analysis after the mice experienced after 0.5, 1.0 and 2.0 h inhalation. The blood plasma of three mice were collected in heparin tube and the volatile compounds were isolated and concentrated by the C-18 solid phase extraction column with methanol and bidistilled water mixture (60:40) as the solvent. The recovery analysis was increased up to 90 %. The reproducibility of extraction methods might be qualified with the indicated value of the variation coefficient less than 15 %. This method successfully detected all the volatile compounds in the blood plasma which the concentration was relatively low (μg/mL or ppm).