Simultaneous Estimation of Moexipril and Hydrochlorthiazide in Tablet Dosage Form by RP-HPLC Method

Abstract

A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous estimation of moexipril and hydrochlorthiazide in tablets. A column having 150 mm × 4.6 mm i.d. in isocratic mode with mobile phase containing acetonitrile:phosphate buffer (45:55; adjusted to pH 3) was used. The flow rate was 0.6 mL/min and effluent was monitored at 215 nm. The retention time (min) and linearity range (μg/mL) for moexipril and hydrochlorthiazide were (4.294, 3.368) and (20-60, 20-60), respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of moexipril and hydrochlorthiazide in tablets.