Determination of Benzoic Acid and Sorbic Acid in Tobacco Sauce by Ultra Performance Liquid Chromatography

Abstract

A rapid and reliable method is presented for the determination of the preservatives (benzoic acid and sorbic acid) in tobacco sauce. The procedure utilizes ultra performance liquid chromatography followed by UV diode array detection for identification and quantitation of the two preservatives. The benzoic acid and sorbic acid were extracted from tobacco sauce samples with methanol/ ammonium acetate buffer solution (25:75) by high speed homogenization and all samples were filtered to remove particulate matter prior to analysis. The ultra performance liquid chromatography determination of the preservatives was performed using a waters ACQUITY UPLC^TM BEH C₁₈ (2.1 × 50 mm, 1.7 mm) column and UV detection at 226 nm for benzoic acid and 258 nm for sorbic acid. The contents of preservative in the sample were be quantized by external standard method. The relative standard derivation of overall intraday variations were less than 1.8 % and the relative standard derivation of interday variations were less than 2.2 %. The standard recoveries (three different concentrations of markers: 0.5, 1.0 and 2.0 mg) were ranged from 97.8-102.4 %.