Comparison of Different Headspace Gas Sampling Methods for the Analysis of Floral Scent from Lilium ‘Siberia’

Abstract

Headspace solid-phase microextraction (HS-SPME) and direct thermal desorption (DTD) coupled to gas chromatography with mass spectrometry (GC/MS) were used to determine the floral scent composition of Lilium 'Siberia'. Three types of fiber coating 100 μm PDMS, 2 cm-50/30 μm DVB/CAR/PDMS and 75 μm CAR/PDMS were employed for solid-phase microextraction sampling and Tenax GR was used as adsorbent for direct thermal desorption. The results demonstrated that the major floral scent components were monoterpenoids (73.78-98.52 %) and benzenoids (1.09-25.98 %). Linalool, (E)- b-ocimene and methyl benzoate were the most major components. Their total relative amounts were 83.28-97.92 %. In the tests, 26 compounds were separated by DTD-GC/MS, more than the other sampling mediums. As direct thermal desorption provided a more flexible sampling site. We considered that direct thermal desorption with Tenax GR absorbent is more suitable for Lilium flower fragrance sampling.