Antifungal and Phytotoxic Activities of Ethyl Acetate Soluble Fraction and Purified Compound (Ethyl-3-hydroxy-5-methoxy-4-methylbenzoate) from Lonicera quinquelocularis

Dilfaraz Khan; Shafiullah Khan; Azim Khan  Khattak; Hazrat Ali; Syed Badshah; Muhammad Akram

doi:10.14233/ajchem.2016.19865

Issue

Vol. 28 No. 9 (2016): Vol 28 Issue 9

Issue Published : June 1, 2016

This work is licensed under a Creative Commons Attribution 4.0 International License.

Antifungal and Phytotoxic Activities of Ethyl Acetate Soluble Fraction and Purified Compound (Ethyl-3-hydroxy-5-methoxy-4-methylbenzoate) from Lonicera quinquelocularis

https://doi.org/10.14233/ajchem.2016.19865

Dilfaraz Khan

Institute of Chemical Sciences, Gomal University, Dera Ismail Khan 29050, Pakistan

Shafiullah Khan

Institute of Chemical Sciences, Gomal University, Dera Ismail Khan 29050, Pakistan

Azim Khan Khattak

Institute of Chemical Sciences, Gomal University, Dera Ismail Khan 29050, Pakistan

Hazrat Ali

Institute of Chemical Sciences, Gomal University, Dera Ismail Khan 29050, Pakistan

Syed Badshah

Institute of Chemical Sciences, Gomal University, Dera Ismail Khan 29050, Pakistan

Muhammad Akram

Medicinal Botanical Centre, PCSIR Lab Complex, Peshawar, Pakistan

Corresponding Author(s) : Dilfaraz Khan

dilfarazkhan@gu.edu.pk

Asian Journal of Chemistry, Vol. 28 No. 9 (2016): Vol 28 Issue 9
Article Published : June 2, 2016

Abstract

Chromatographic separation and purification of ethyl acetate fractions of Lonicera quinquelocularis resulted in the isolation of a new compound namely ethyl-3-hydroxy-5-methoxy-4-methylbenzoate. The ethyl acetate fractions extract of Lonicera quinquelocularis and isolated compound ethyl-3-hydroxy-5-methoxy-4-methylbenzoate were screened for antifungal and phytotoxic activities. The ethyl acetate fraction was effective against Aspergillus flavus (55.5 %) and Aspergillus niger (61 %). Furthermore, the new purified compound and ethyl acetate soluble fraction showed phototoxic effect as well.

Keywords

Lonicera quinquelocularis Antifungal Phytotoxic

Khan, D., Khan, S., Khattak, A. K., Ali, H., Badshah, S., & Akram, M. (2016). Antifungal and Phytotoxic Activities of Ethyl Acetate Soluble Fraction and Purified Compound (Ethyl-3-hydroxy-5-methoxy-4-methylbenzoate) from Lonicera quinquelocularis. Asian Journal of Chemistry, 28(9), 2001–2003. https://doi.org/10.14233/ajchem.2016.19865

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References

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References

P.J. Houghton, Z. Boxu and Z. Xisheng, Phytother. Res., 7, 384 (1993); doi:10.1002/ptr.2650070513.

R. Puupponen-Pimia, L. Nohynek, C. Meier, M. Kahkonen, M. Heinonen, A. Hopia and K.M. Oksman-Caldentey, J. Appl. Microbiol., 90, 494 (2001); doi:10.1046/j.1365-2672.2001.01271.x.

M.P. Kahkonen, A.I. Hopia and M. Heinonen, J. Agric. Food Chem., 49, 4076 (2001); doi:10.1021/jf010152t.

J.Z. Shi, X.F. Chen and L. Wan, China J. China Mater. Med., 24, 363 (1999).

J. Wang, X.-Z. Zhao, Q. Qi, L. Tao, Q. Zhao, R. Mu, H.-Y. Gu, M. Wang, X. Feng and Q.-L. Guo, Food Chem. Toxicol., 47, 1716 (2009); doi:10.1016/j.fct.2009.04.034.

H.J. Yoo, H.J. Kang, Y.S. Song, E.H. Park and C.J. Lim, J. Pharm. Pharmacol., 60, 779 (2008); doi:10.1211/jpp.60.6.0014.

K. Machida, J. Asano and M. Kikuchi, Photochemistry, 39, 111 (1995); doi:10.1016/0031-9422(94)00853-L.

F. Bailleul, A. Leveau and M. Durand, J. Nat. Prod., 44, 573 (1981); doi:10.1021/np50017a011.

I. Souzu and H. Mitsuhashi, Tetrahedron Lett., 10, 2725 (1969); doi:10.1016/S0040-4039(01)88253-8.

I. Souzu and H. Mitsuhashi, Tetrahedron Lett., 11, 191 (1970); doi:10.1016/S0040-4039(01)97672-5.

I. Ali, D. Khan, F. Ali, H. Bibi and A. Malik, J. Chem. Soc. Pak., 35, 139 (2013).

S. Kumar, O.P. Sati, V.D. Semwal, M. Nautiyal, S. Sati and Y. Takeda, Phytochemistry, 53, 499 (2000); doi:10.1016/S0031-9422(99)00426-4.

D. Khan, M. Afzal, S. Woodward and S. Khan, Rec. Nat. Prod., 8, 121 (2014).

D. Khan, H.U. Khan, F. Khan, S. Khan, S. Badshah, A.S. Khan and A. Samad, PLoS ONE, 9, e94952 (2014), PLoS ONE, 9, 1 (2014); doi:10.1371/journal.pone.0094952.

D. Khan, S. Khan, S. Badshah, H. Ali, H. Ullah, Z. Muhammad and S. Woodward, Nat. Prod. Res., 30, 74 (2015); doi:10.1080/14786419.2015.1036267.

M.A. Gyamfi, M. Yonamine and Y. Aniya, Gen. Pharmacol., 32, 661 (1999); doi:10.1016/S0306-3623(98)00238-9.

J.L. McLaughlin, Proceedings of NIH Workshop, Bioassay for Discovery of Antitumoral, Antiviral Agents from Natural Sources, vol. 18, 22 (1988).

P.P. Joy, J. Thomas, S. Mathew and B.P. Skaria, Medicinal Plants, Conservation and Livelihoods; In: Tropical Horticulture, Naya Prokash, Calcutta, vol. 2, p. 449-632 (2001).

B. Tepe, D. Daferera, A. Sokmen, M. Sokmen and M. Polissiou, Food Chem., 90, 333 (2005); doi:10.1016/j.foodchem.2003.09.013.

E. Ernst, Ann. Intern. Med., 136, 42 (2002); doi:10.7326/0003-4819-136-1-200201010-00010.

W. Tassaneeyakul, E. Razzazi-Fazeli, S. Porasuphatana and J. Bohm, Mycopath, 158, 239 (2004); doi:10.1023/B:MYCO.0000041892.26907.b4.

Issue

Vol. 28 No. 9 (2016): Vol 28 Issue 9

Antifungal and Phytotoxic Activities of Ethyl Acetate Soluble Fraction and Purified Compound (Ethyl-3-hydroxy-5-methoxy-4-methylbenzoate) from Lonicera quinquelocularis

Corresponding Author(s) : Dilfaraz Khan

Abstract

Keywords

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