LC-MS/MS Method for Simultaneous Determination of Pteryxin and Bergapten in Rat Plasma for Pharmacokinetic Study

Abstract

A sensitive LC-MS/MS method for simultaneous determination of pteryxin and bergapten in rat plasma was established to analyze the pharmacokinetics. Plasma samples were extracted using methanol with isoimperatorin as an internal standard (IS). Separation was carried out on a C₁₈ column using a mixture of water and methanol (30:70, v/v) as the mobile phase. The effluent was monitored by UV detector at 300 nm and at a flow rate of 1 mL/min and 30 ºC. The linearity ranges of proposed method were 0.132 5-53.00 μg/L for pteryxin and 8.12-162.4 μg/L for bergapten. The intra-day and inter-day RSD of the assay method for the two components were less than 10 % and mean recovery was within the 83.24-91.27 % range. The method was found to be precise, accurate and specific during the study and was successfully used to analyze the pharmacokinetic of pteryxin and bergapten. Pharmacokinetic data of pteryxin and its metabolites were obtained with this method after oral administration.