Characterization of Partial cDNA Sequence for Gnetum gnemon Resveratrol Synthase Encoding Gene

Abstract

Gnetum gnemon is known to be a potential natural source for resveratrol. Gnetum gnemon resveratrol synthase encoding gene then was considered to be one of the targets to be cloned. This work describes the characterization of partial cDNA sequence coding for resveratrol synthase by RT-PCR amplification. A pair gene specific primers (5'-GCAACCGTCCTGGCAATCGC-3') and (5'-GTTCCACCTGCGAAGC AGCC-3') was successfully used to amplify a 450 bp fragment that codes for a partial length of Gnetum gnemon. The DNA sequences were shown to share a high level of identity to resveratrol synthase from other plants at about 70 % identity. This homology is higher compared with a common 66 % identitiy level of resveratrol synthase among known resveratrol-producing plants.