Detection of Ethyl Parathion Using Microcantilever Biosensor
Changguo Xue
School of Materials Science and Engineering, Anhui University of Science and Technology, Huainan 232001, Anhui Province, P.R. China ; Key Laboratory of Mechanical Behaviour and Design of Material of Chinese Academy of Sciences, University of Science and Technology of China, Hefei, P.R. China
Tiegui Nan
College of Agronomy and Biotechnology, China Agricultural University, Beijing, P.R. China
Shangquan Wu
Key Laboratory of Mechanical Behaviour and Design of Material of Chinese Academy of Sciences, University of Science and Technology of China, Hefei, P.R. China
Zhen Cao
College of Agronomy and Biotechnology, China Agricultural University, Beijing, P.R. China
Qingchuan Zhang
Key Laboratory of Mechanical Behaviour and Design of Material of Chinese Academy of Sciences, University of Science and Technology of China, Hefei, P.R. China
Baomin Wang
College of Agronomy and Biotechnology, China Agricultural University, Beijing, P.R. China
Corresponding Author(s) : Changguo Xue
chgxue@foxmail.com
Asian Journal of Chemistry,
Vol. 24 No. 9 (2012): Vol 24 Issue 9
A microcantilever biosensor has been developed for label-free detection of ethyl parathion. Through self-assembled monolayer (SAM) method, anti ethyl parathion antibody was immobilized on the gold surface of the microcantilever for the measurement of ethyl parathion. The deflection of the microcantilever corresponding to different ethyl parathion concentration was real time monitored by optical lever technique. The experimental results of non-competitive enzyme-linked immunosorbent assay (ELISA) indicate that anti ethyl parathion antibody on the gold surface can keep the activity. Deflection of microcantilever is linear to ethyl parathion concentration from 500-2000 ng/mL. The limit of detection is 500 ng/mL.
Xue, C., Nan, T., Wu, S., Cao, Z., Zhang, Q., & Wang, B. (2012). Detection of Ethyl Parathion Using Microcantilever Biosensor. Asian Journal of Chemistry, 24(9), 3951–3953. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/9510
