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Vol. 24 No. 9 (2012): Vol 24 Issue 9

Denaturation of Protein Using Microcantilever Sensor

Changguo Xue
School of Materials Science and Engineering, Anhui University of Science and Technology, Huainan 232001, Anhui Province, P.R. China ; Key Laboratory of Mechanical Behaviour and Design of Material of Chinese Academy of Sciences, University of Science and Technology of China, Hefei, P.R. China
Yanhua Teng
School of Materials Science and Engineering, Anhui University of Science and Technology, Huainan 232001, Anhui Province, P.R. China
Shangquan Wu
Key Laboratory of Mechanical Behaviour and Design of Material of Chinese Academy of Sciences, University of Science and Technology of China, Hefei, P.R. China
Qingchuan Zhang
Key Laboratory of Mechanical Behaviour and Design of Material of Chinese Academy of Sciences, University of Science and Technology of China, Hefei, P.R. China
Mingxu Zhang
School of Materials Science and Engineering, Anhui University of Science and Technology, Huainan 232001, Anhui Province, P.R. China

Corresponding Author(s) : Changguo Xue

chgxue@foxmail.com

Asian Journal of Chemistry, Vol. 24 No. 9 (2012): Vol 24 Issue 9

Abstract

A real-time detection denaturation of the trypsin using microcantilever sensor platform with optical lever technique was reported. The trypsin was indirectly immobilized on the cantilever gold surface by thiol self-assembled monolayer and the activated carboxy group of the thiol by EDC/NHS. Consequently, the deflections of the microcantilever corresponding to the process of injecting different concentration of GuHCl were generated.

Keywords

Microcantilever Denaturation Surface stress
(1)
Xue, C.; Teng, Y.; Wu, S.; Zhang, Q.; Zhang, M. Denaturation of Protein Using Microcantilever Sensor. ajc 2012, 24, 3948-3950.
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