Enzyme Kinetics in Crowded Cellular Environment

Abstract

This study reports, the Michaelis-Menten scheme of enzyme-network kinetics in crowded environment based on a particle-based stochastic model. The effects of crowding, cooperative reaction from enzyme-network and size of substrates are investigated. The diffusion behaviour is computed and compared under different obstacle volume fraction and different size of substrate. We find the crowded environment embeded in enzyme-network greatly increases the compartmentalization of substrate with big size, leading to the obvious increase of probability of rebind of released substrate to its initial enzyme and decrease to other enzymes. When volume fraction of obstacle is increased, the survival probability of small substrate is decreased while it increases in big substrate case. Time dependent reaction rate coefficient is studied in different time scale.