Chemical Composition of the Essential Oil and Petroleum Ether Extract of Korean Pinus densiflora Leaves

Abstract

The composition of the leaves of Pinus densiflora essential oil and petroleum ether extract were examined by gas chromatographic-mass spectroscopic analysis (GC-MS). The leaves of P. densiflora on hydrodistillation gave 0.30 % essential oil yield (mL/100 g) of a fresh weight basis. Thirty seven and fourty compounds comprising 96.52 and 99.44 % of the total peak area were identified in the essential oil and petroleum ether extract, respectively. The major components of essentials oils were bicyclo[2.2.1]heptan-2-ol 1,7,7-trimethyl acetate (11.64 %), trans-a-caryophyllene (20.08 %), a-humulene (5.23 %), naphthalene 1,2,4a, 5,6,8a-hexahydro-4,7-dimethyl-1-(1-methylethyl)-(1a, 4aa, 8aa)-(3.89 %), d-cadinene (3.57 %), caryophyllene oxide (7.49 %), t-muurolol (8.57 %), (-)-spathulenol (3.53), t-cadinol (3.30 %), a-cadinol (3.36 %). The major components of petroleum ether extract were acetic acid butyl ester (17.25 %), a-pinene (2.64 %), bicyclo[2.2.1]heptan-2-ol 1,7,7-trimethyl acetate (13.95 %), sabinene (4.48 %), a-selinene (6.18 %), napthalene 1,2,3, 4,4a,5,6,8a-octahydro-7-methyl-4-methylene-1-(1-methylethyl)-(1a, 4aa, 8aa)-(6.77 %), (-)-spathulenol (5.58 %), caryophyllene oxide (8.10 %). The identity of components of essential oil and petroleum ether extract were confirmed on the basis of retention time, mass and supplemented library.