Composition of the Essential Oil, Neutral Volatile Oil and Petroleum Ether Extract of Korean Perilla (Perilla frutescens Britton) Leaves

Abstract

The composition of the essential oil, neutral volatile oil and petroleum ether extract from the leaves of Perilla frutescens of Korea were analyzed and identified by gas chromatographicmass spectrometry (GC-MS). The essential and neutral volatile oil yields (mL/100 g DW) were for the 0.40 % and 0.30 % respectively. Thirty six and thirty seven compounds comprising 100 % and 99.71 % of the total peak area were identified in essential oil and neutral volatile oil, respectively. Thirty nine compounds comprising 99.85 % of the total peak area were identified in petroleum ether extract. The major components of the essential and neutral volatile oil were trans caryophyllene (1.87 %), perilla ketone (90.19 %), (Z,E)-1,3,6,10-dodecatetraene-3,7,11- trimethyl (1.76 %) and perilla ketone (83.33 %), 1H-3a, 7-methanoazulene 2,3,4,7,8,8a-hexahydro-3,6,8,8-tetramethyl (1.48 %), piperazine 2,5-dibutyl-3,6-dimethyl (1.09 %), 9-octadecenoic acid (2-phenyl-1,3-dioxolan-4-yl) methyl ester (1.13 %), methyl propyl pyridine-2-carboxylate-5-carboxythiolate (3.48 %), respectively. The major componentes of the petroleum ether extract were acetic acid butyl ester (15.64 %), pentanoic acid 4-methyl (2.05 %), 1-octen-3-ol (2.36 %), eicosane (1.75 %), hexadecane (1.03 %), (E,Z)-2,6-nonadienal (1.34 %), phenyl ethyl alcohol (1.15 %), 3-ethyl-5-methyl-1-heptyne-3-ol (1.64 %), perilla ketone (41.89 %), 2(3H)-furanone dihydro-5-methyl-5-(2-methylpropyl) (3.93 %), phenol 2-methoxy-4-(2-propenyl, eugenol) (11 %), cyclohexanol 1-propyl (2.56 %), n-hexadecanoic acid (1.51 %), ethyl linoleolate (2.55 %). The identity of components of essential oil, neutral volatile oil and petroleum ether extract was confirmed on the basis of retention time, mass and supplemented library of NIST, USA. The neutral volatile oil and petroleum ether extract constituents are identified for the first time in P. frutescens leaves.