Determination of in vitro Produced Cichoriin from Callus Culture of Cichorium intybus L.

Abstract

Reversed-phase high performance liquid chromatography (HPLC) has been developed for determination of in vitro produced Cichoriin from callus culture of Cichorium intybus L. Cichoriin was obtained from exhaustive methanolic extract of lyophillized callus tissue. Extract was chromatographed with ethyl acetate as mobile phase on a silica gel column. Ethyl acetate elution was followed by preparative thin layer chromatography. The extract was also processed by reversed-phase HPLC using C₁₈ column. The proposed method was validated in accordance with the International Conference on Harmonization (ICH) Q2 (R1) validation guidelines by determining its selectivity, linearity, accuracy and precision. Based on the results, method is an effective choice to analyze this molecule.