Sensitive Determination and Pharmacokinetic Study of Amlodipine in Human Serum by LC-ESI/MS/MS
N. Li
Department of Chemistry and Anhui Key Lab for Biomass Clean Energy, University of Science and Technology of China, Hefei 230026, P.R. China
*Corresponding author: Fax: +86 551 3606689; Tel: +86 551 3600659; E-mail: xyu@ustc.edu.cn
Y. Xu
Department of Chemistry and Anhui Key Lab for Biomass Clean Energy, University of Science and Technology of China, Hefei 230026, P.R. China
Y.N. Luo
Department of Chemistry and Anhui Key Lab for Biomass Clean Energy, University of Science and Technology of China, Hefei 230026, P.R. China
*Corresponding author: Fax: +86 551 3606689; Tel: +86 551 3600659; E-mail: xyu@ustc.edu.cn
J.J. Sun
Department of Chemistry and Anhui Key Lab for Biomass Clean Energy, University of Science and Technology of China, Hefei 230026, P.R. China
*Corresponding author: Fax: +86 551 3606689; Tel: +86 551 3600659; E-mail: xyu@ustc.edu.cn
Y.Y. Zhai
Department of Chemistry and Anhui Key Lab for Biomass Clean Energy, University of Science and Technology of China, Hefei 230026, P.R. China
*Corresponding author: Fax: +86 551 3606689; Tel: +86 551 3600659; E-mail: xyu@ustc.edu.cn
Q.X. Guo
Department of Chemistry and Anhui Key Lab for Biomass Clean Energy, University of Science and Technology of China, Hefei 230026, P.R. China
*Corresponding author: Fax: +86 551 3606689; Tel: +86 551 3600659; E-mail: xyu@ustc.edu.cn
Corresponding Author(s) : Y. Xu
xyu@ustc.edu.cn
Asian Journal of Chemistry,
Vol. 24 No. 6 (2012): Vol 24 Issue 6
A sensitive LC-ESI/MS/MS method was developed and validated to determine the concentrations of amlodipine in human serum. Amlodipine and the internal standard (IS) omeprazole were extracted from 200 μL of human serum by liquid-liquid extraction, using ethyl acetate as the extraction solvent. Sample analysis was performed by reversed-phase LC-MS/MS with electrospray ionization in positive ion mode, using selected reaction monitoring. The mobile phase composed of methanol and 0.15 % ammonium acetate in water (78 : 22, v/v) at a flow rate of 0.2 mL/min. The linearity ranged from 0.2 to 12.8 ng/mL. The extraction recoveries of amlodipine ranged from 90.68 to 105.27 %. The method was successfully used to pharmacokinetic study of amlodipine after an oral administration of 10 mg amlodipine tablets to 19 healthy volunteers.
Keywords
AmlodipineLC-ESI/MS/MSPharmacokinetic
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Li, N., Xu, Y., Luo, Y., Sun, J., Zhai, Y., & Guo, Q. (2012). Sensitive Determination and Pharmacokinetic Study of Amlodipine in Human Serum by LC-ESI/MS/MS. Asian Journal of Chemistry, 24(6), 2415–2418. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/9146
