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Vol. 24 No. 6 (2012): Vol 24 Issue 6

Investigation on the Potential use of the Mimetic Peroxidase-Catalyzed Reaction of Hydrogen Peroxide and Catechol in Aqueous-Organic Mixtures

J.W. Liu
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, P.R. China
Y.L. Wang
Institute of Applied Chemistry, Hebei North University, Zhangjiakou 075000, P.R. China
K. Sun
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, P.R. China
J. Chen
College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, P.R. China

Corresponding Author(s) : J.W. Liu

liujiwei@zjnu.cn

Asian Journal of Chemistry, Vol. 24 No. 6 (2012): Vol 24 Issue 6

Abstract

The reaction between hydrogen peroxide and catechol catalyzed by mimetic peroxidase hemin has been adapted to determination of trace amounts of H2O2. The present work employs hemin as mimetic peroxidase to catalyze the oxidation of catechol by H2O2. Its intermediate is obtained in aqueous-organic two phases and the absorption peaks at 260 nm. The catalytic optimum conditions for hemin-H2O2-catechol two phases system were studied. As low as 2 × 10-6 M H2O2 could be detected with a linear range from 3 × 10-6 M to 1 × 10-4 M via spectrophotometry.

Keywords

Hemin Mimetic enzyme Aqueous-organic mixtures
Liu, J., Wang, Y., Sun, K., & Chen, J. (2012). Investigation on the Potential use of the Mimetic Peroxidase-Catalyzed Reaction of Hydrogen Peroxide and Catechol in Aqueous-Organic Mixtures. Asian Journal of Chemistry, 24(6), 2380–2382. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/9137
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