in vivo Evaluation of Noxious Stimulus Induced Pain and Inflammation in Mice and Rats Using Methanolic Extract of Galphimia glauca Leaf

Baba Shankar Rao Garige; Srisailam Keshetti; Uma Maheshwara Rao Vattikuti

doi:10.14233/ajchem.2016.19839

Issue

Vol. 28 No. 8 (2016): Vol 28 Issue 8

Issue Published : April 30, 2016

This work is licensed under a Creative Commons Attribution 4.0 International License.

in vivo Evaluation of Noxious Stimulus Induced Pain and Inflammation in Mice and Rats Using Methanolic Extract of Galphimia glauca Leaf

https://doi.org/10.14233/ajchem.2016.19839

Baba Shankar Rao Garige

Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Hyderabad-501 301, India

Srisailam Keshetti

University College of Pharmaceutical Sciences, Satavahana University, Karimnagar-505 002, India

Uma Maheshwara Rao Vattikuti

Department of Pharmacy, C.M.R. Group of Institutions, Hyderabad-501 401, India

Corresponding Author(s) : Srisailam Keshetti

ksrisailam@yahoo.com

Asian Journal of Chemistry, Vol. 28 No. 8 (2016): Vol 28 Issue 8
Article Published : April 30, 2016

Abstract

This study assesses the analgesic and anti-inflammatory properties of methanolic extract of Galphimia glauca leaf using in vivo models. The dose range of 100, 200 and 400 mg/kg b.w was administered orally for assessing analgesic and anti-inflammatory activity respectively. Further studies were conducted for determining the involvement of central and peripheral receptor actions in the analgesic activity of the extract by pre-challenging it with naloxone and acetic acid respectively. The LD₅₀ of the extract was found to be > 2000 mg/kg b.w. The leaf extract at 400 mg/kg dose exhibited significant (P £ 0.001) and dose-dependent analgesic activity. It also exhibited central and peripheral analgesic actions when treated with naloxone and acetic acid respectively. However, the extract of leaf has shown anti-inflammatory activity, which is not comparable to that of standard drug. The results revealed that the methanolic extract of Galphimia glauca leaf has potential in terms of analgesic properties.

Keywords

Acetic acid Carrageenan Diclofenac sodium Formalin Granuloma Galphimia glauca leaf

Rao Garige, B. S., Keshetti, S., & Rao Vattikuti, U. M. (2016). in vivo Evaluation of Noxious Stimulus Induced Pain and Inflammation in Mice and Rats Using Methanolic Extract of Galphimia glauca Leaf. Asian Journal of Chemistry, 28(8), 1815–1819. https://doi.org/10.14233/ajchem.2016.19839

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References

C. Anderson, Contrib. Univ. Mich. Herb., 25, 82 (2007).
M.G. Campos, E. Toxqui, J. Tortoriello, M.V. Oropeza, H. Ponce, M.H. Vargas and L.M. Montaño, J. Ethnopharmacol., 74, 7 (2001); doi:10.1016/S0378-8741(00)00322-6.
L. Aguilar-Santamaría, G. Ramírez, A. Herrera-Arellano, A. Zamilpa, J.E. Jiménez, D. Alonso-Cortés, E.I. Cortés-Gutiérrez, N. Ledesma and J. Tortoriello, J. Ethnopharmacol., 109, 35 (2007); doi:10.1016/j.jep.2006.06.013.
OECD Guideline for Testing Chemicals, OECD/OCDE 423, pp. 1-14 (2001).
P.K. Mukherjee, Quality Control of Herbal Drugs: An Approach to Evaluation of Botanicals, Business Horizons Pharmaceutical Publications, New Delhi, pp. 529-533 (2002).
P. Methods, A Guide to Modern Techniques of Plant Analysis, Harbone J.B, Springer Verlag, Berlin, pp.107-200 (2005).
I.O. Ishola, A.J. Akindele and O.O. Adeyemi, J. Ethnopharmacol., 135, 55 (2011); doi:10.1016/j.jep.2011.02.024.
Z.A. Zakaria, H. Patahuddin, A.S. Mohamad, D.A. Israf and M.R. Sulaiman, J. Ethnopharmacol., 128, 42 (2010); doi:10.1016/j.jep.2009.12.021.
N.O.A. Omisore, C.O. Adewunmi, E.O. Iwalewa, B.T. Ngadjui, J. Watchueng, B.M. Abegaz and J.A.O. Ojewole, J. Ethnopharmacol., 95, 7 (2004); doi:10.1016/j.jep.2004.05.022.
H.O. Mbagwa, R.A. Anene and O.O. Adeyemi, Nig. Q. J. Hosp. Med., 17, 35 (2007).
M. Shibata, T. Ohkubo, H. Takahashi and R. Inoki, Pain, 38, 347 (1989); doi:10.1016/0304-3959(89)90222-4.
M. William Carey, N.V. Rao, B.R. Kumar and G.K. Mohan, J. Ethnopharmacol., 130, 179 (2010); doi:10.1016/j.jep.2010.04.023.
R. Koster, M. Anderson and E.J. De Beer, Fed. Proc., 18, 412 (1959).
O.O. Adeyemi, S.O. Okpo and O. Okpaka, J. Ethnopharmacol., 90, 45 (2004); doi:10.1016/j.jep.2003.09.021.
P. Laupattarakasem, T. Wangsrimongkol, R. Surarit and C. Hahnvajanawong, J. Ethnopharmacol., 108, 349 (2006); doi:10.1016/j.jep.2006.05.029.
C.A. Winter, E.A. Risley and G.W. Nuss, Exp. Biol. Med., 111, 544 (1962); doi:10.3181/00379727-111-27849.
T.A. Aziz, B.H. Marouf, N.M.A. Mohamood and S.A. Hussain, Global J. Pharmacol., 5, 86 (2011).
Z.A. Zakaria, Z.D.F. Abdul Ghani, R.N.S. Raden Mohd. Nor, H.K. Gopalan, M.R. Sulaiman, A.M. Mat Jais, M.N. Somchit, A.A. Kader and J. Ripin, J. Nat. Med., 62, 179 (2008); doi:10.1007/s11418-007-0224-x.
S.O. Okpo, F. Fatokun and O.O. Adeyemi, J. Ethnopharmacol., 78, 207 (2001); doi:10.1016/S0378-8741(01)00318-X.
B. Ibrahim, A. Sowemimo, A. van Rooyen and M. Van de Venter, J. Ethnopharmacol., 141, 282 (2012); doi:10.1016/j.jep.2012.02.032.
C. Punzón, A. Alcaide and M. Fresno, Int. Immunopharmacol., 3, 1293 (2003); doi:10.1016/S1567-5769(03)00117-6.

References

C. Anderson, Contrib. Univ. Mich. Herb., 25, 82 (2007).

M.G. Campos, E. Toxqui, J. Tortoriello, M.V. Oropeza, H. Ponce, M.H. Vargas and L.M. Montaño, J. Ethnopharmacol., 74, 7 (2001); doi:10.1016/S0378-8741(00)00322-6.

L. Aguilar-Santamaría, G. Ramírez, A. Herrera-Arellano, A. Zamilpa, J.E. Jiménez, D. Alonso-Cortés, E.I. Cortés-Gutiérrez, N. Ledesma and J. Tortoriello, J. Ethnopharmacol., 109, 35 (2007); doi:10.1016/j.jep.2006.06.013.

OECD Guideline for Testing Chemicals, OECD/OCDE 423, pp. 1-14 (2001).

P.K. Mukherjee, Quality Control of Herbal Drugs: An Approach to Evaluation of Botanicals, Business Horizons Pharmaceutical Publications, New Delhi, pp. 529-533 (2002).

P. Methods, A Guide to Modern Techniques of Plant Analysis, Harbone J.B, Springer Verlag, Berlin, pp.107-200 (2005).

I.O. Ishola, A.J. Akindele and O.O. Adeyemi, J. Ethnopharmacol., 135, 55 (2011); doi:10.1016/j.jep.2011.02.024.

Z.A. Zakaria, H. Patahuddin, A.S. Mohamad, D.A. Israf and M.R. Sulaiman, J. Ethnopharmacol., 128, 42 (2010); doi:10.1016/j.jep.2009.12.021.

N.O.A. Omisore, C.O. Adewunmi, E.O. Iwalewa, B.T. Ngadjui, J. Watchueng, B.M. Abegaz and J.A.O. Ojewole, J. Ethnopharmacol., 95, 7 (2004); doi:10.1016/j.jep.2004.05.022.

H.O. Mbagwa, R.A. Anene and O.O. Adeyemi, Nig. Q. J. Hosp. Med., 17, 35 (2007).

M. Shibata, T. Ohkubo, H. Takahashi and R. Inoki, Pain, 38, 347 (1989); doi:10.1016/0304-3959(89)90222-4.

M. William Carey, N.V. Rao, B.R. Kumar and G.K. Mohan, J. Ethnopharmacol., 130, 179 (2010); doi:10.1016/j.jep.2010.04.023.

R. Koster, M. Anderson and E.J. De Beer, Fed. Proc., 18, 412 (1959).

O.O. Adeyemi, S.O. Okpo and O. Okpaka, J. Ethnopharmacol., 90, 45 (2004); doi:10.1016/j.jep.2003.09.021.

P. Laupattarakasem, T. Wangsrimongkol, R. Surarit and C. Hahnvajanawong, J. Ethnopharmacol., 108, 349 (2006); doi:10.1016/j.jep.2006.05.029.

C.A. Winter, E.A. Risley and G.W. Nuss, Exp. Biol. Med., 111, 544 (1962); doi:10.3181/00379727-111-27849.

T.A. Aziz, B.H. Marouf, N.M.A. Mohamood and S.A. Hussain, Global J. Pharmacol., 5, 86 (2011).

Z.A. Zakaria, Z.D.F. Abdul Ghani, R.N.S. Raden Mohd. Nor, H.K. Gopalan, M.R. Sulaiman, A.M. Mat Jais, M.N. Somchit, A.A. Kader and J. Ripin, J. Nat. Med., 62, 179 (2008); doi:10.1007/s11418-007-0224-x.

S.O. Okpo, F. Fatokun and O.O. Adeyemi, J. Ethnopharmacol., 78, 207 (2001); doi:10.1016/S0378-8741(01)00318-X.

B. Ibrahim, A. Sowemimo, A. van Rooyen and M. Van de Venter, J. Ethnopharmacol., 141, 282 (2012); doi:10.1016/j.jep.2012.02.032.

C. Punzón, A. Alcaide and M. Fresno, Int. Immunopharmacol., 3, 1293 (2003); doi:10.1016/S1567-5769(03)00117-6.

Issue

Vol. 28 No. 8 (2016): Vol 28 Issue 8

in vivo Evaluation of Noxious Stimulus Induced Pain and Inflammation in Mice and Rats Using Methanolic Extract of Galphimia glauca Leaf

Corresponding Author(s) : Srisailam Keshetti

Abstract

Keywords

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