Determination the content of Plumbagin in Plumbago zeylanica L. During Different Collected Periods by RP-HPLC

Abstract

The content of plumbagin in leaves and branches of Plumbago zeylanica collected during different periods grown in Guangxi, China, were determined using RP-HPLC with methanol and H₂O (70:30) as mobile phase and UV detection at 254 nm. Linear range over 0.1220-1.2198 mg/mL of plumbagin was obtained. Recovery was within 98.6-102.8 %. The content in leaves and branches of Plumbago zeylanica is highest in October when the plant growth is most thriving and biggest in size. It is therefore considered that October is the best time for collecting leaves and branches.