RP-HPLC Estimation of Clebopride in Pure and Tablet Dosage Form

Abstract

A rapid, accurate, sensitive and precise reverse phase high performance liquid chromatographic method was developed for the estimation of clebopride in pure and tablet dosage form. Separation of the drug was carried out on a reverse phase C₁₈ column using a mobile phase consisting of 0.1 % phosphoric acid and acetonitrile in the ratio of 70:30 v/v. The flow rate was 1 mL/min. The detection of clebopride has done at a wavelength of 272 nm. The linearity was found in the range of 25-200 μg/mL with a correlation coefficient of 0.999. The proposed method was validated for its sensitivity, linearity, accuracy and precision. The developed RP-HPLC method was successfully applied for the quantitative determination of clebopride in tablets.