Antineoplastic Effect of Hydrolyzate from Oyster on Human Nasopharyngeal Carcinoma Cells

Abstract

To investigate the effects of hydrolyzate from oyster on antineoplastic of human nasopharyngeal carcinoma cell line CNE-1. The inhibited effects of the bioactive peptide, ploysaccharides and small molecule compounds from Guangxi oyster on the growth of nasopharyngeal carcinoma CNE-1 were tested by MTT. To observe the effect of oyster ploysaccharides on the change of morphocytology by means of HE staining method. The bioactive peptide with molecular weight under 3000Da of oyster can inhibit human CNE-1 cell culture, which IC₅₀ is 120.00 mg/L. The inhibiting effect of bioactive peptide with molecular weight under 3000 Da after 250 mg/L treatment, the inhibitory rate of cellular growth was 48.26 %. The ploysaccharides of oyster can inhibit human CNE-1 cell culture, which IC₅₀ is 138.25 mg/L.The inhibiting effect of ploysaccharides after 250 mg/L treatment, the inhibitory rate of cellular growth was 61.78 %. Scalaradial, the small molecule compound of oyster can inhibit human CNE-1 cell culture, which IC₅₀ is 138.29 mg/L. The inhibiting effect of Scalaradial after 250 mg/L treatment, the inhibitory rate of cellular growth was 64.02 %. Under a microscope, the cells showed morphological changes significantly such as the cell nucleus shrinking, syrup diminishing, karyopyknosis occurring, cytoplasm concentrating and the number of cells reducing. Bioactive peptide, ploysaccharides and small molecule compounds from Guangxi oyster have a growing inhibiting effect on CNE-1 cells at a certain consistency. It also has a synergistic effect on differentiation of tumor cells.