Extraction and Purification of Bromelain

Abstract

Bromelain is a crude protein extract obtained from pineapple fruit and stem, which comprises a variety of proteolytic enzymes. It is applied for reduction of muscle and tissue inflammation, alleviation of bronchitis and sinusitis, as an aid in digestion and for relieving symptoms of gout. The most important application of bromelain includes meat tenderization. Pineapple was peeled and crushed to extract the juice containing soluble bromelain enzyme. Further processing included, precipitation, dialysis and ion exchange chromatography of enzyme. Ion exchange elutes (pure enzyme) reveal the activity of bromelain by titrimetric enzyme assay method.