Analysis of Floral Volatiles by Using Headspace-Solid Phase Microextraction: A Review

Abstract

Headspace solid phase microextraction (HS-SPME) is a relatively new technique for analysis of floral volatiles. Volatile components emitted from the flowers are pre-concentrated on solid phase microextraction fiber by using field sampling manual holder. The volatiles are identified by GC-MS and percentage composition determined by GC-FID. Solid phase microextraction is a technique that can be used to analyze the fragrance of flowers in different time intervals without solvent treatment. The technique is simple and pre-concentration is easily achieved in the field without sophisticated experimental arrangement. The basic concepts of solid phase microextraction along with the existing methods of floral extraction are discussed. Headspace solid phase microextraction analysis of the volatile compounds emitted by plants and plant products in general and by flowers in particular has been reviewed. The volatile composition of live and picked flowers of Jasminum sambac, Michelia champaca, Murraya paniculata and Quisqualis indica are discussed.