Analysis of Semen Euphorbiae Frostlike Powders by HPLC Fingerprint
Corresponding Author(s) : Zhen-Ling Zhang
Asian Journal of Chemistry,
Vol. 25 No. 17 (2013): Vol 25 Issue 17 page 10011-10014
In present work, the analysis method of semen euphorbiae frostlike powders with HPLC fingerprint was established. The high performance liquid chromatography was applied. The chromatographic column was Agilent Zorbax-C18 (4.6 × 250 mm, 5 μm) column. The flow phase was acetonitrile:water = 61:39; the flow velocity was: 1 mL min-1, the detection wavelength was 280 nm and the column temperature was 40 °C. The fingerprints of semen euphorbiae frostlike powders were established and 7 common peaks were identified. Fingerprint of semen euphorbiae frostlike powder samples and that of the control group shared a similarity of at least 0.9 and can be used in the qualitative identification of semen euphorbiae frostlike powders.
Download CitationEndnote/Zotero/Mendeley (RIS)