HPLC-MS/MS Based Profiling of Antimutagenic Agents of Digera muriacata Leaf

Muhammad Yasir; Bushra Sultana; Haq Nawaz Bhatti; Muhammad Shahid

doi:10.14233/ajchem.2016.19243

Issue

Vol. 28 No. 6 (2016): Vol 28 Issue 6

Issue Published : February 29, 2016

This work is licensed under a Creative Commons Attribution 4.0 International License.

HPLC-MS/MS Based Profiling of Antimutagenic Agents of Digera muriacata Leaf

https://doi.org/10.14233/ajchem.2016.19243

Muhammad Yasir

Department of Chemistry, University of Agriculture, Faisalabad-38040, Pakistan

Bushra Sultana

Department of Chemistry, University of Agriculture, Faisalabad-38040, Pakistan

Haq Nawaz Bhatti

Department of Chemistry, University of Agriculture, Faisalabad-38040, Pakistan

Muhammad Shahid

Department of Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan

Corresponding Author(s) : Bushra Sultana

bushrasultana2005@yahoo.com

Asian Journal of Chemistry, Vol. 28 No. 6 (2016): Vol 28 Issue 6
Article Published : February 29, 2016

Abstract

The current study was aimed to characterize antimutagenic agents of Digera muriacata by high performance liquid chromatography coupled with mass spectrometry. Extraction procedure was optimized for better recovery of antimutagenic agents using acidified methanol (0.5, 1.0 and 2.0 N) and aqueous methanol (70 %, 50 % and 30 % v/v) to get hydrolyzed and non-hydrolyzed extracts, respectively. Both types of extracts were found to be non-mutagens with hydrolyzed extracts depicting higher antimutagenicity (70.63 ± 0.69 % for TA98 and 73.98 ± 0.70 % for TA100) than those of non-hydrolyzed extracts (68.25 ± 0.69 % for TA98 and 68.29 ± 1.22 % for TA100). Hence, optimized hydrolyzed extract was further characterized for its bioactives by LC-MS/MS analysis which showed the presence of ten compounds including kaempferol-O-dirhamnoside, 5-O-feruloylquinic acid, malvidine-3-O-glucoside, etc. These results potentially recommended vast applications of Digera muriacata in medicinal formulations for curing various diseases.

Keywords

Digera muriacata LCMS/MS Antimutagenic activity

Yasir, M., Sultana, B., Bhatti, H. N., & Shahid, M. (2016). HPLC-MS/MS Based Profiling of Antimutagenic Agents of Digera muriacata Leaf. Asian Journal of Chemistry, 28(6), 1205–1209. https://doi.org/10.14233/ajchem.2016.19243

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References

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References

M.P. Iqbal, S.F. Kazim and N.M. Ali, Pak. J. Pharm. Sci., 19, 282 (2007).

A. Aktumsek, G. Zengin, G.O. Guler, Y.S. Cakmak and A. Duran, Food Chem., 141, 91 (2013); doi:10.1016/j.foodchem.2013.02.092.

H. Li, Z. Deng, H. Zhu, C. Hu, R. Liu, J.C. Young and R. Tsao, Food Res. Int., 46, 250 (2012); doi:10.1016/j.foodres.2011.12.014.

A.H. Ebrahimabadi, Z. Djafari-Bidgoli, A. Mazoochi, F.J. Kashi and H. Batooli, Food Contr., 21, 1173 (2010); doi:10.1016/j.foodcont.2010.01.014.

T. Guo, L. Wei, J. Sun, C.L. Hou and L. Fan, Food Chem., 127, 1634 (2011); doi:10.1016/j.foodchem.2011.02.030.

D.R. Kammerer, J. Kammerer, R. Valet and R. Carle, Food Res. Int., 65, 2 (2014); doi:10.1016/j.foodres.2014.06.012.

D.T. Santos, R. Vardanega and M.A. De Almeida, Pharmacognosy Rev., 8, 88 (2014);doi:10.4103/0973-7847.134231.

S. Sadiq, M. Imran, M.N. Hassan, M. Iqbal, Y. Zafar and F.Y. Hafeez, LWT- Food Sci. Technol., 59, 204 (2014); doi:10.1016/j.lwt.2014.05.018.

N. Sharma and R. Vijayvergia, Int. J. Pharm. Sci. Rev. Res., 20, 114 (2013).

M.F.A. Razak and K.E. Aidoo, Asian J. Pharm. Clin. Res., 4, 23 (2011).

S. Mosovska, M. Mikulasova, L. Brindzova, L. Valík and L. Mikusova, Food Chem. Toxicol., 48, 1483 (2010); doi:10.1016/j.fct.2010.03.015.

M. Imran, A.M. Revol-Junelles, M. de Bruin, C. Paris, E. Breukink and S. Desobry, J. Mic. Meth., 95, 107 (2013); doi:10.1016/j.mimet.2013.07.009.

A. Rohman, S. Riyanto, N. Yuniarti, W.R. Saputra, R. Utami and W. Mulatsih, Int. Food Res. J., 17, 97 (2010).

A. Aremu, M. Moyo, S. Amoo and J. Van Staden, S. Afr. J. Bot., 87, 95 (2013); doi:10.1016/j.sajb.2013.03.002.

J. Chukwujekwu, S. Amoo and J. Van Staden, J. Ethnopharmacol., 148, 975 (2013); doi:10.1016/j.jep.2013.06.003.

R. Mulaudzi, A. Ndhlala, M. Kulkarni, J. Finnie and J.V. Staden, J. Ethnopharmacol., 146, 173 (2013); doi:10.1016/j.jep.2012.12.026.

R. Paterson and N. Lima, Food Contr., 35, 329 (2014); doi:10.1016/j.foodcont.2013.07.024.

C. Carazzone, D. Mascherpa, G. Gazzani and A. Papetti, Food Chem., 138, 1062 (2013); doi:10.1016/j.foodchem.2012.11.060.

A. Rameshkumar, T. Sivasudha, R. Jeyadevi, B. Sangeetha, G.S.B. Aseervatham and M. Maheshwari, Food Res. Int., 50, 94 (2013); doi:10.1016/j.foodres.2012.09.035.

I.M. Abu-Reidah, D. Arraez-Roman, R. Quirantes-Pine, S. Fernandez-Arroyo, A. Segura-Carretero and A. Fernández-Gutiérrez, Food Res. Int., 46, 108 (2012); doi:10.1016/j.foodres.2011.11.026.

J.Y. Zhang, Q. Zhang, N. Li, Z.J. Wang, J.Q. Lu and Y.J. Qiao, Talanta, 104, 1 (2013); doi:10.1016/j.talanta.2012.11.012.

A. Arya, S.C. Cheah, C.Y. Looi, H. Taha, M.R. Mustafa and M.A. Mohd, Food Chem. Toxicol., 50, 4209 (2012); doi:10.1016/j.fct.2012.08.012.

B. Pfundstein, S.K. El Desouky, W.E. Hull, R. Haubner, G. Erben and R.W. Owen, Phytochemistry, 71, 1132 (2010); doi:10.1016/j.phytochem.2010.03.018.

J. Sun, F. Liang, Y. Bin, P. Li and C. Duan, Molecules, 12, 679 (2007); doi:10.3390/12030679.

Issue

Vol. 28 No. 6 (2016): Vol 28 Issue 6

HPLC-MS/MS Based Profiling of Antimutagenic Agents of Digera muriacata Leaf

Corresponding Author(s) : Bushra Sultana

Abstract

Keywords

Full Article

Download Citation

References

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