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Analysis of the Chemical Constituents of BaZhen Decoction in Rat Urine by HPLC-ESI/MS
Corresponding Author(s) : Wenlan Li
Asian Journal of Chemistry,
Vol. 25 No. 15 (2013): Vol 25 Issue 15
Abstract
High performance liquid chromatography/mass spectrometry (HPLC-ESI/MS) method was used to study BaZhen decoction and its metabolites in rat urine. 30 of major fragment ion peaks were marked in the total ion chromatogram of urine containing BaZhen decoction. According to the molecular weight and characteristic fragmentation information, 20 peaks were identified, containing 7 prototype constituents: Glycyol, glyuranolide, paeoniflorin, ginsenoside Rb2, liquiritin, PGG, ginsenoside Rd; and 13 metabolites: p-Hydroxycinnamic acid, 5-FDCA, pinicolic acid, 3a-hydroxyglycyrrhehetic acid, paeonimetabolin I, M1, Compound K, M2, tumulosic acid, M3, n-g-hydroxy-butyl phthalide, M4, Ginsenoside Rh1. The result shows that the final metabolites in rat urine of BaZhen decoction, which is quite valuable for the further studies on effective constituents and the effecting mechanism as well as for clinical application of Bazhen decoction.
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- W.L. Li, Q.C. Dai, Y.B. Ji, Z. Sun, J. Du and J. Bai, Chin Pharm., 21, 1022 (2009).
- Z. Chun, X. Luo, D.H. Chen, M.Y. Yu, Y.F. Cheng and Z.R. Yang, J. Biomed. Eng., 21, 727 (2004).
- H.P. Pan, Chin Trad. Patent Med., 25, 90 (2003).
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- H.M. Wen, K.J. Guo, W.L. Xing and H.M. Bian, J. Nanjing Univ. TCM, 23, 96 (2007).
- L. Yang, S.J. Xu, X. Zeng, Z.F. Wu, Y.H. Deng, Y.M. Liu, S.G. Deng and R.M. Ou, Chem. J. Chin. Univ., 27, 1042 (2006).
- L. Yang, S.J. Xu, X. Zeng, Z.F. Wu, Y.H. Deng, Y.M. Liu, S.G. Deng and R.M. Ou, Acta Pharm. Sin., 41, 742 (2006).
- X.F. Zhao, H.W. Kong, J.S. Wang and G.W. Xu, World Sci. Technol.- Modern Trad. Chin. Med., 11, 153 (2009).
- F.Q. Guo, L.F. Huang and S.Y. Zhao, Chin. J. Chromatogr., 25, 43 (2007).
- B.L. Qu, H.Y. Wu, F.L. Ren, W.F. Yu and J.Z. Wang, Food Sin. Technol., 34, 92 (2009).
- N. Yang, S.Y. Qiao and F.H. Meng, J. Chin. Mass Spectrom, 31, 153 (2010).
- Y. Liu, R.B. Shi, B. Liu and M. Dong, J. Beijing Univ. TCM, 31, 334 (2008).
- N.J. Yu, J.F. Guo, Y.M. Zhao and Y.X. Zhang, Chin. J. Anal. Chem., 3, 866 (2004).
- L. Zhang, D.S. Xu and Y. Feng, Chin. Mater. Med., 32, 1789 (2007).
- J.J. Zhou, G.R. Xie and F.J. Yan, Traditional Chinese Medicines Molecular Structures, Natural Sources and Applications, Beijing, pp. 365, 533 (2004).
References
W.L. Li, Q.C. Dai, Y.B. Ji, Z. Sun, J. Du and J. Bai, Chin Pharm., 21, 1022 (2009).
Z. Chun, X. Luo, D.H. Chen, M.Y. Yu, Y.F. Cheng and Z.R. Yang, J. Biomed. Eng., 21, 727 (2004).
H.P. Pan, Chin Trad. Patent Med., 25, 90 (2003).
X.R. Ran, H.H. Yang, Q.L. Liang, J. Chen, Y.M. Wang, G.A. Luo, P. Li, K.M. Li and Y.B. Chen, Chem. J. Chin. Univ., 28, 250 (2007).
H.M. Wen, K.J. Guo, W.L. Xing and H.M. Bian, J. Nanjing Univ. TCM, 23, 96 (2007).
L. Yang, S.J. Xu, X. Zeng, Z.F. Wu, Y.H. Deng, Y.M. Liu, S.G. Deng and R.M. Ou, Chem. J. Chin. Univ., 27, 1042 (2006).
L. Yang, S.J. Xu, X. Zeng, Z.F. Wu, Y.H. Deng, Y.M. Liu, S.G. Deng and R.M. Ou, Acta Pharm. Sin., 41, 742 (2006).
X.F. Zhao, H.W. Kong, J.S. Wang and G.W. Xu, World Sci. Technol.- Modern Trad. Chin. Med., 11, 153 (2009).
F.Q. Guo, L.F. Huang and S.Y. Zhao, Chin. J. Chromatogr., 25, 43 (2007).
B.L. Qu, H.Y. Wu, F.L. Ren, W.F. Yu and J.Z. Wang, Food Sin. Technol., 34, 92 (2009).
N. Yang, S.Y. Qiao and F.H. Meng, J. Chin. Mass Spectrom, 31, 153 (2010).
Y. Liu, R.B. Shi, B. Liu and M. Dong, J. Beijing Univ. TCM, 31, 334 (2008).
N.J. Yu, J.F. Guo, Y.M. Zhao and Y.X. Zhang, Chin. J. Anal. Chem., 3, 866 (2004).
L. Zhang, D.S. Xu and Y. Feng, Chin. Mater. Med., 32, 1789 (2007).
J.J. Zhou, G.R. Xie and F.J. Yan, Traditional Chinese Medicines Molecular Structures, Natural Sources and Applications, Beijing, pp. 365, 533 (2004).