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Vol. 25 No. 15 (2013): Vol 25 Issue 15

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Determination of Monoamine Neurotransmitters in Brains of Paraquat-Induced Mouse by HPLC with Electrochemical Detection

https://doi.org/10.14233/ajchem.2013.14856
Siyuan Bi
School of Public Health, Hebei Medical University, Shijiazhuang 050017, P.R. China
Wei Wang
School of Public Health, Hebei Medical University, Shijiazhuang 050017, P.R. China
Kaoqi Lian
School of Public Health, Hebei Medical University, Shijiazhuang 050017, P.R. China
Xiangdong Xu
School of Public Health, Hebei Medical University, Shijiazhuang 050017, P.R. China
Lingmei Niu
School of Public Health, Hebei Medical University, Shijiazhuang 050017, P.R. China
Hongmei Shi
School of Public Health, Hebei Medical University, Shijiazhuang 050017, P.R. China
Weijun Kang
School of Public Health, Hebei Medical University, Shijiazhuang 050017, P.R. China

Corresponding Author(s) : Weijun Kang

kangwj@hebmu.edu.cn; huangpuchangshou@163.com

Asian Journal of Chemistry, Vol. 25 No. 15 (2013): Vol 25 Issue 15

Abstract

A liquid chromatography-electrochemical detection method for the quantification of dopamine, 3,4-dihydroxyphenyl acetic acid, homovanillic acid, 3-methoxy-4-hydroxy phenylglycol, 5-hydroxytryptamine and 5-hydroxyindole-3-acetic acid was presented. The neurotransmitters were analyzed by an liquid chromatography on a C18 column with an electrochemical detection. Brain samples with 1 mL of 5 % sulfosalicylic acid were homogenized and centrifuged, then for analysis. This method exhibited excellent linearity for all of the analytes with regression coefficients higher than 0.99. The limit of detection values for dopamine, 3,4-dihydroxyphenyl acetic acid, homovanillic acid, 3-methoxy-4-hydroxy phenylglycol, 5-hydroxytryptamine and 5-hydroxyindole-3-acetic acid were 2.2, 3.0, 1.7, 3.7, 0.9 and 1.0 ng/mL, respectively. The precision results were expressed as relative standard deviation, ranging from 2.1 to 3.8 % for intra-day and from 2.8 to 4.8 % for the inter-day. The recoveries were ranged from 88.6 to 94.5 % for intra-day and from 86.6 to 93.9 % for the inter-day. This novel HPLC-ECD approach is precise, highly sensitive, specific and sufficiently simple.

Keywords

Monoamine neurotransmitters Paraquat Mouse brain HPLC Electrochemistry detector
Bi, S., Wang, W., Lian, K., Xu, X., Niu, L., Shi, H., & Kang, W. (2013). Determination of Monoamine Neurotransmitters in Brains of Paraquat-Induced Mouse by HPLC with Electrochemical Detection. Asian Journal of Chemistry, 25(15), 8593–8596. https://doi.org/10.14233/ajchem.2013.14856
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