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Vol. 25 No. 15 (2013): Vol 25 Issue 15

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Extraction and Determination of Chloramphenicol in Meat Using 1-Butyl-3-methylimidazolium Chloride-K2HPO4 Aqueous Two-Phase System Coupled with HPLC

https://doi.org/10.14233/ajchem.2013.14847
D.Y. Jiang
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, P.R. China ; College of Chemistry, Jilin Normal University, Siping 136000, P.R. China
J. Han
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, P.R. China
C.L. Yu
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, P.R. China
Y.F. Li
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, P.R. China
L. Wang
College of Chemistry, Jilin Normal University, Siping 136000, P.R. China
Y.S. Yan
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, P.R. China ; State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, P.R. China

Corresponding Author(s) : D.Y. Jiang

Asian Journal of Chemistry, Vol. 25 No. 15 (2013): Vol 25 Issue 15

Abstract

An ionic liquid-salt aqueous two-phase system (ILATPS) consisting of 1-butyl-3-methylimidazolium chloride ([C4mim]Cl) and K2HPO4, which is a simple, green and rapid sample pretreatment technique was used for the simultaneous separation, concentration and analysis of chloramphenicol (CAP) coupled with high-performance liquid chromatography. The influence factors on partitions of chloramphenicol were studied, including the types of ionic liquids and salts, the concentration of salt and chloramphenicol, temperature, pH and the volume of ionic liquid. In [C4mim]Cl-K2HPO4 ILATPS, the extraction efficiency of chloramphenicol increased with the volume of [C4mim]Cl increase and decreased with temperature increase. Under the optimal conditions, the average extraction efficiency of chloramphenicol in [C4mim]Cl-K2HPO4 ILATPS was up to 95 %. This method has been satisfactorily applied to the analysis of chloramphenicol in meat samples with the detection limit of 0.23 ng g-1 and relative standard deviation (RSD) of 1.27 % (n = 7). The recovery of chloramphenicol was 94.4-107 % from meat samples with a RSD of 0.38-1.47 %, showing that this method was satisfactory for the separation of antibiotics.

Keywords

Ionic liquid Aqueous two-phase system Chloramphenicol HPLC Extraction
Jiang, D., Han, J., Yu, C., Li, Y., Wang, L., & Yan, Y. (2013). Extraction and Determination of Chloramphenicol in Meat Using 1-Butyl-3-methylimidazolium Chloride-K2HPO4 Aqueous Two-Phase System Coupled with HPLC. Asian Journal of Chemistry, 25(15), 8579–8583. https://doi.org/10.14233/ajchem.2013.14847
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