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Vol. 25 No. 15 (2013): Vol 25 Issue 15

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Rhamnus triquetra: A Valuable Source of Natural Antioxidants to Shield from Oxidative Stress

https://doi.org/10.14233/ajchem.2013.14843
Farhana Mazhar
Department of Chemistry, Government College University, Lahore-54000, Pakistan
Muhammad Jahangir
Department of Chemistry, Government College University, Lahore-54000, Pakistan
Muhammad Athar Abbasi
Department of Chemistry, Government College University, Lahore-54000, Pakistan
Aziz-ur Rehman
Department of Chemistry, Government College University, Lahore-54000, Pakistan
Syed Anam Ilyas
Department of Chemistry, Government College University, Lahore-54000, Pakistan
Farah Khalid
Department of Chemistry, Government College University, Lahore-54000, Pakistan
Raisa Khanum
Department of Chemistry, Government College University, Lahore-54000, Pakistan
Saba Jamil
Department of Chemistry, Government College University, Lahore-54000, Pakistan
Shaheen Kausar
Department of Chemistry, Government College University, Lahore-54000, Pakistan
Muhammad Ajaib
Department of Botany, Government College University, Lahore-54000, Pakistan

Corresponding Author(s) : Muhammad Athar Abbasi

atrabbasi@yahoo.com

Asian Journal of Chemistry, Vol. 25 No. 15 (2013): Vol 25 Issue 15

Abstract

The aim of this study was to examine the in vitro antioxidant potential of different fractions of Rhamnus triquetra. The methanolic extract of this plant was dissolved in distilled water and successively partitioned with n-hexane, chloroform, ethyl acetate and n-butanol. Phyto-chemical screening revealed the presence of alkaloids, terpenoids, tannins, phenolics, sugars, saponins and flavonoids. The antioxidant potential of crude methanolic extract, all these fractions and remaining aqueous fraction was evaluated by different methods i.e., DPPH free radical scavenging activity, total antioxidant activity, ferric reducing antioxidant power assay, ferric thiocyanate assay and total phenolic content assay. All the fractions exhibited significant antioxidant potential. The results revealed that ethyl acetate soluble fraction showed the highest % inhibition of DPPH radical (92.01 ± 0.21) having an IC50 value of 7.59 ± 0.65 μg/mL, total antioxidant activity value (1.840 ± 0.08), highest ferric reducing antioxidant power value of (2137.7 ± 0.58 μg/mL) and inhibition of lipid peroxidation (61.94 ± 1.17 %). The chloroform fraction showed the highest total phenolic content value (121.5 ± 1.10 μg/g) as compared to other fractions.

Keywords

Rhamnus triquetra Antioxidant potential Oxidative stress Organic fractions Lipid peroxidation
Mazhar, F., Jahangir, M., Athar Abbasi, M., Rehman, A.- ur, Anam Ilyas, S., Khalid, F., … Ajaib, M. (2013). Rhamnus triquetra: A Valuable Source of Natural Antioxidants to Shield from Oxidative Stress. Asian Journal of Chemistry, 25(15), 8569–8573. https://doi.org/10.14233/ajchem.2013.14843
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References
  1. A. Matkowski, Biotechnol. Advan., 26, 548 (2008).
  2. B. Halliwell, Am. J. Med., 91, 1422 (1991).
  3. J. Nordberg and E.S.J. Arner, Free Radic. Biol. Med., 31, 1287 (2001).
  4. S. Tayyaba, M.A. Abbasi, A.U. Rehman, R. Tauheeda, K.M. Khan, V.U. Ahmad, M. Ashraf, B. Hussain and M. Ajaib, J. Chem. Soc. Pak., 34, 442 (2012).
  5. J. Javanmardi, C. Stushnoff, E. Locke and J.M. Vivanco, Food Chem., 83, 547 (2003).
  6. B.N. Singh, B.R. Singh, R.L. Singh, D. Prakash, B.K. Sarma and H.B. Singh, Food Chem. Toxicol., 47, 1161 (2009).
  7. S. Walter and G. Richard, Am. J. Bot., 91, 1627 (2004).
  8. W.C. Ko, M.C. Chen, S.H. Wang, Y.H. Lai, J.H. Chen and C.N. Lin, Planta Med., 69, 310 (2003).
  9. M. Ajaib, Z. Khan, N. Khan and M. Wahab, Pak. J. Bot., 42, 1407 (2010).
  10. N.P. Manandhar and S. Manandhar, Plants and People of Nepal Timber Press Incorporated Portland, Oregon, p. 393 (2002).
  11. D. Frohne and H.J. Pfander, A Colour Atlas of Poisonous Plant, Wolfe Publishing Ltd., p. 291 (1984).
  12. G.E. Trease and W.C. Evans, Pharmacognosy, Bailliere Tindall, London, edn. 13, pp. 176-180 (1989).
  13. B.O. Obadoni and P.O. Ochuko, Global J. Pure Appl. Sci., 8, 203 (2001).
  14. R. Tauheeda, M.A. Abbasi, A.U. Rehman, S. Tayyaba, M. Ajaib and K.M. Khan, Pak. J. Pharm. Sci., 25, 679 (2012).
  15. P. Prieto, M. Pineda and M. Aguilar, Anal. Biochem., 269, 337 (1999).
  16. I.E.F. Benzie and J.J. Strain, Anal. Biochem., 239, 70 (1996).
  17. H.P.S. Makkar, M. Bluemmel, N.K. Borowy and K. Becker, J. Sci. Food Agric., 61, 161 (1993).
  18. P. Valentao, E. Fernandes, F. Carvalho, P.B. Andrade, R.M. Seabra and M.L. Bastos, J. Agric. Food Chem., 50, 4989 (2002).
  19. D.J. Huang, H.J. Chen, C.D. Lin and Y.H. Lin, Bot. Bull. Acad. Sin., 46, 99 (2008).
  20. B. Archana, N. Dasgupta and B. De, Food Chem., 90, 727 (2005).
  21. S. Miladi and M. Damak, J. Soc. Chim. Tunisie, 10, 101 (2008).
  22. I.F.F. Benzie, Clin. Biochem., 29, 111 (1996).
  23. C.A. Rice-Evans, N.J. Miller, P.M. Bramley and J.B. Pridham, Free Radic. Res., 22, 375 (1995).
  24. E.A. Decker, Nutr. Rev., 55, 396 (1997).
  25. J. Sun, Y.F. Chu, X. Wu and R.H. Liu, J. Agric. Food Chem., 50, 449 (2002).
  26. J.S.J. Kim and M.J. Kim, J. Med. Plants Res., 4, 674 (2010)
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