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Synthesis of Biologically Important Angiotensin-II and Angiotensin-IV Peptides by Using 4-(2',4'-Dimethoxypenyl-Fmoc-Aminomethyl)phenoxy Resin
Corresponding Author(s) : R. Selvam
Asian Journal of Chemistry,
Vol. 25 No. 15 (2013): Vol 25 Issue 15
Abstract
In peptide chemistry, there is the goal to produce pure biologically active peptides with the highest possible efficiency, even its possible by chemical synthesis purity and yield as a major problem. In this work, we focus to overcome above difficulty by novel synthetic approach. Angiotensin an oligopeptide is a hormone and a powerful dipsogen, which exhibits a wide range of biological activities, the 8-amino acid sequence Angiotensin-II and 6-amino acid sequence. Angiotensin-IV were synthesized by using 4-(2',4'-dimethoxyphenyl-Fmoc-aminomethyl) phenoxy resin, the modified Fmoc-chemistry and effective hydroxybenzenetriazole, N,N'-diisopropylcarbodiimide coupling and activation methods was used. The yield and chromatographic purities were compared.
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- URL, http://www.ornl.gov/TechResources/Human_Genome/home.html
- R. Rudolph and H. Lilie, FASEB J., 10, 49 (1996).
- G.B. Fields, R.H. Angeletti, S.A. Carr, A.J. Smith, J.T. Stults, L.C. Williams and J.D. Young, In ed.: J.W. Crabb, Techniques in Protein Chemistry V: pp. 501-507 (1994).
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- G.B. Fields, R.H. Angeletti, L.F. Bonewald, W.T. Moore, A.J. Smith, J.T. Stults and L.C. Williams, In ed.: J.W. Crabb, Techniques in Protein Chemistry VI, pp. 539-546 (1995).
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- M. Bodanszky, Principles of Peptide Synthesis; Springer Verlag: New York (1984).
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References
URL, http://www.ornl.gov/TechResources/Human_Genome/home.html
R. Rudolph and H. Lilie, FASEB J., 10, 49 (1996).
G.B. Fields, R.H. Angeletti, S.A. Carr, A.J. Smith, J.T. Stults, L.C. Williams and J.D. Young, In ed.: J.W. Crabb, Techniques in Protein Chemistry V: pp. 501-507 (1994).
J. Galpin, Amino Acids, Peptides and Proteins; The Royal Society ofChemistry, Burlington House, London, Vo1. 16, pp. 272- 341 (1985).
B. Merrifield, Science, 232, 341 (1986).
L.P. Miranda and P.F. Alewood, Accelerated Chemical Synthesis of Peptides and Small Proteins, Proceedings of the National Academy of Sciences of the United States of America, 96, pp. 1181-1186 (1999).
G.B. Fields, R.H. Angeletti, L.F. Bonewald, W.T. Moore, A.J. Smith, J.T. Stults and L.C. Williams, In ed.: J.W. Crabb, Techniques in Protein Chemistry VI, pp. 539-546 (1995).
E.L. Boulpaep and W.F. Boron, Medical Physiology: a Cellular and Molecular Approach. St. Louis, Mo: Elsevier Saunders, p. 771 (2005).
N. Basso and N.A. Terragno, Hypertension, 38, 1246 (2001).
M. Bodanszky, Principles of Peptide Synthesis; Springer Verlag: New York (1984).
G. Barany and R.B. Merrifield, In eds.: E. Gross and J. Meienhoferm, In: The Peptides: Analysis, Synthesis and Biology, Academic Press: New York, pp. 1-228 (1980).
E. Atherton and R.C. Shepard, Solid Phase Peptide Synthesis-A Practical Approachusing the Fmoc-Polyamide Techinique, IRL Press, Oxford (1984)