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Immobilization of the Protease of Carica papaya on Amberlite CG-400
Corresponding Author(s) : H. Bokhari
Asian Journal of Chemistry,
Vol. 25 No. 13 (2013): Vol 25 Issue 13
Abstract
Immobilization of the protease of Carica papaya was carried out on amberlite CG-400. The percentage of immobilization was found to be 54 %. A continuous immobilized enzyme proteolytic system was developed by packing a column with Amberlite CG-400 using citrate phosphate buffer pH 7. The column was partly chocked due to adsorption of casein with Amberlite CG-400, which led to reduction in the flow rate. The products of proteolysis collected in the form of 5 mL fractions were assayed by Lowry's method. The system operated successfully for proteolysis on continuous basis. The life span of the enzyme immobilized on Amberlite CG-400 was 27 d.
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