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Vol. 28 No. 5 (2016): Vol 28 Issue 5

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Phytochemicals, Central Nervous System and Analgesic Activity of Ethyl Acetate Extract of Casearia vareca Roxb. Leaves

https://doi.org/10.14233/ajchem.2016.19606
Bonoranjan Phukan
Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh-786 004, India
Atul Kumar
Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh-786 004, India
Bibhuti Bhushan Kakoti
Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh-786 004, India

Corresponding Author(s) : Bonoranjan Phukan

bonoranjanphukan@gmail.com

Asian Journal of Chemistry, Vol. 28 No. 5 (2016): Vol 28 Issue 5

Abstract

The present study involves the preliminary investigation of phytochemical constituents, acute toxicity study and to evaluate depressant effects on central nervous system of the ethyl acetate extract (EACV) of leaves of Casearia vareca Roxb. (Flacourtiaceae) in Swiss albino mice. The Casearia vareca leaves powder was defatted with petroleum ether and then extracted with ethyl acetate in Soxhlet apparatus. The vacuum dried ethyl acetate leaves extract of Casearia vareca (EACV) obtained was subjected to various phytochemical as well as pharmacological screening. The ethyl acetate extract at the doses of 100, 200 and 400 mg/kg body weight was subjected to evaluation of central nervous system and analgesic activities in experimental animal models. Central nervous system activity was evaluated by the observation of behavioural profiles such as awareness and alertness, touch response, righting reflex, pinna reflex, grip strength, pain response, sound response, corneal reflex and spontaneous motor activity in mice at various doses. Analgesic activity was evaluated by the chemical (acetic acid induced writhing response), thermal (hot plate, tail immersion) and mechanical (tail clip) method. The extract exhibited the central nervous system depressant activity at 200 and 400 mg/kg per oral (p.o.) route and the activities were well comparable with the standard drug, diazepam (5 mg/kg, i.p.) The analgesic activity of the extract was evaluated using acetic acid induced writhing response, hot plate, tail immersion and tail clip method. The extract showed analgesic activity that was comparable with standard drug, acetyl salicylic acid and morphine. The data were verified as statistically significant by using one way analysis of variance (ANOVA).

Keywords

Casearia vareca Spontaneous motor activity Analgesic Writhing Hot plate Tail immersion
Phukan, B., Kumar, A., & Kakoti, B. B. (2016). Phytochemicals, Central Nervous System and Analgesic Activity of Ethyl Acetate Extract of Casearia vareca Roxb. Leaves. Asian Journal of Chemistry, 28(5), 1127–1132. https://doi.org/10.14233/ajchem.2016.19606
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