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Vol. 25 No. 13 (2013): Vol 25 Issue 13

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HPLC Determination of Aflatoxins in Wheat Grains Collected from Central Areas of the Punjab, Pakistan

https://doi.org/10.14233/ajchem.2013.15836
Sabahat Ahsan
Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad-38000, Pakistan
Ijaz Ahmad Batti
Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad-38000, Pakistan
Zaib Hussain
Institute of Chemistry, University of the Punjab, Lahore-54590, Pakistan
Shazia Anwar Bukhari
Department of Chemistry, Government College University, Faisalabad-38000, Pakistan
Syed Ali Raza Naqvi
Department of Chemistry, Government College University, Faisalabad-38000, Pakistan
Zulfiqar Ali Khan
Department of Chemistry, Government College University, Faisalabad-38000, Pakistan
Muhammad Rafique Asi
Nuclear Institute of Agriculture and Biology, Jhang Road, Faisalabad-38000, Pakistan

Corresponding Author(s) : Sabahat Ahsan

drsabahat.ahsan@hotmail.com

Asian Journal of Chemistry, Vol. 25 No. 13 (2013): Vol 25 Issue 13

Abstract

Occurrence of aflatoxins (B1, B2, G1 and G2) in samples of wheat from different localities in Faisalabad division, Punjab, Pakistan was determined with latest technique by using HPLC-UV and HPLC-FLD in isocratic mode using MycoSep # 226 columns. Total 40 samples were analyzed each taken from urban, semi-urban and rural areas. Contaminated samples, their percentage, mean value of aflatoxins, highest aflatoxin B1 and total B1 and B2, G1 and G2 was determined. Samples collected from semi-urban areas were found more contaminated 36 μg kg-1 (limit 2-25 μg kg-1 FDA, USA).

Keywords

Aflatoxins HPLC-FLD Cereals Validation Food Wheat
Ahsan, S., Ahmad Batti, I., Hussain, Z., Anwar Bukhari, S., Ali Raza Naqvi, S., Ali Khan, Z., & Rafique Asi, M. (2013). HPLC Determination of Aflatoxins in Wheat Grains Collected from Central Areas of the Punjab, Pakistan. Asian Journal of Chemistry, 25(13), 7463–7466. https://doi.org/10.14233/ajchem.2013.15836
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