An Evaluation of Haemolytic and Antioxidant Potential of Kalanchoe pinnata Leaves

Abstract

The present study was carried out to determine the haemolytic and antioxidant potential of Kalanchoe pinnata leaves. Antioxidant activity was determined by measuring reducing potential, DPPH radical scavenging (IC₅₀), total phenolic contents and total flavonoid contents. The % yield of leaves extracts were found to be in the range of 2.2 to 3.8 %. The plant leaves contained appreciable levels of total phenolic contents (2.28-6.24 GAE mg/g of dry extract) and total flavonoid contents (2.3-4.5 CE mg/g of dry extract). DPPH radical scavenging was found to be in the range of 35.4-70.12 μg/mL. The haemolytic effect of the plant leaves was found in range of 0.82-4.96 %. The antioxidant activity of plant extracts was also studied using sunflower oil as an oxidative substrate and found that it stabilized the oil. The results of the present investigation demonstrated significant (p < 0.05) variations in the results. The correlation between the results of different antioxidant assays and oxidation parameters of oil indicated that leaves extracts, exhibited considerable TPC, TFC and scavenging power, were also more potent for enhancing the oxidative stability of sunflower oil.