Phytochemical Screening and Antioxidant Evaluations of Different Fractions of Argyrolobium roseum

Abstract

The present investigations were carried out to establish the medicinal value of Argyrolobium roseum synonym Cytisus roseum. It is a medicinal plant, belongs to family Leguminoseae. The antioxidant potentials of the different fractions of A. roseum were screened by using ABTS^•+ assay, DPPH free radical scavenging activity, ferric reducing antioxidant power (FRAP) assay, total antioxidant activity by phosphomolybdenum method, total phenolic contents determination, total flavonoid contents and metal chelating activity of ethyl acetate, n-hexane, chloroform, methanol and aqueous fractions. ABTS^•+ assay results reveal that ethyl acetate soluble fraction demonstrated the good antioxidant activity having the highest Trolox equivalent antioxidant capacity (TEAC) value i.e., 152.43 ± 0.43 mM/μL Trolox equivalent and the highest total flavonoid contents having total flavonoid content value 931.91 ± 0.06 mg/g QE, n-hexane soluble fraction showed the highest percentage of metal chelating activity 61.85 ± 0.9 % bound iron and highest total phenolic contents 792.5 ± 0.55 gallic acid equivalent mg/g whereas aqueous extract indicated the ferric reducing antioxidant power value 70.48 g/mL. It was concluded from antioxidant screening that ethyl acetate soluble fraction was rich in strong radical scavenging active components but chloroform soluble fraction and aqueous extract were valuable resources of phenolics and flavonoids.