Evaluation of Antioxidant and Cytotoxicity Studies of Clerodendrum inerme

Abstract

Clerodendrum inerme has been identified as a source of natural antioxidants. The aim of this work was to check the antioxidant activity of C. inerme stem by measuring total phenolic contents, total flavonoid contents, DPPH free radical scavenging assay, reducing power and percentage inhibition of linoleic acid peroxidation. Total phenolic contents and total flavonoid contents values were found to be in the range (0.89-1.89 mg/100 g GAE) and (3.18-5.68 mg/100 g CE) respectively. The IC₅₀ and percentage inhibition of linoleic acid peroxidation values of C. inerme extracts ranged from (24.1-81 μg/mL) and (41-72 %) respectively. Peroxide value, free fatty acid and para-anisidine values were also studied by stabilizing the canola oil as oxidation substrate. The results showed that methanol extract of C. inerme stem showed better antioxidant activity. The cytotoxicity studies carried out by haemolytic activity of plant extracts against the human erythrocytes (RBCs) in vitro was studied and found to be in the range (1.41-5.75 %) in different extracts.