Determination of Fusarium Mycotoxin Beauvericin in Bombyx batryticatus by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Abstract

Fusarium Mycotoxin beauvericin has been proven to be a potent antibiotic compound useful for bio-control and a potential antifungal and anticancer agent for human. The aim of this study was to determine the level of Fusarium mycotoxin beauvericin in Bombyx batryticatus using LC-MS/MS and observe its content changes in production process within 12 days. The method yielded satisfactory precision, stability and repeatability (RSD: 0.63 %, 0.96 %, 1.16 %). Average recovery of three spiked levels was 100.3 % with RSD 4.54 %. The level of beauvericin changes with a certain law in dead silkworm bodies. Fusarium mycotoxin beauvericin content was almost zero in the first four days, then extended increasing and reached its maximum in 10^th day, which was high up to 43.08 μg/g. The trend of Fusarium mycotoxin beauvericin level declined from 10^th day. This is the first study to report the content of Fusarium mycotoxin beauvericin in B. batryticatus.