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Vol. 28 No. 5 (2016): Vol 28 Issue 5

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Antioxidant and Anti-Inflammatory Activities of Ethyl Ether Extract from Paeonia lactiflora Pall. Flowers

https://doi.org/10.14233/ajchem.2016.19615
Ying-Shan Jin
College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, Jiangsu Province, P.R. China
Jinchuan Chen
College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, Jiangsu Province, P.R. China
Yinzhe Jin
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, P.R. China
Chunxia Li
College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, Jiangsu Province, P.R. China
Manli Chen
College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, Jiangsu Province, P.R. China
Jun Tao
College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu Province, P.R. China

Corresponding Author(s) : Jun Tao

ysjin@yzu.edu.cn; jysh1025@hotmail.com

Asian Journal of Chemistry, Vol. 28 No. 5 (2016): Vol 28 Issue 5

Abstract

Paeonia lactiflora Pall. root is an important traditional Chinese medicine. In order to determine if the flower have any beneficial properties, we investigated antioxidant and anti-inflammatory activities of ethyl ether extract of P. lactiflora Pall. flowers. The results showed that the P. lactiflora Pall. flowers exhibited extremely high antioxidant properties, IC50 values of 1,1-diphenyl-2-pricylhydrazyl free radical scavenging activity was 7.31 mg/mL, which was lower than that of positive control butylated hydroxyanisole and it eliminated approximately 60.51 % of superoxide radicals at 400 μg/mL. Moreover, the P. lactiflora Pall. flowers showed strong total antioxidant activity and reducing power and provided protection against oxidative protein damage induced by hydroxyl radicals. Lipopolysaccharide-activated RAW264.7 cells were used to explore the modulatory role of P. lactiflora Pall. flowers on nitric oxide production and the activation of transcription factors using RT-PCR. P. lactiflora Pall. flowers diminished the production of nitric oxide and the mRNA expression of tumor necrosis factor-a, intedeukin 6, inducible nitric oxide synthase and cyclooxygenase-2.

Keywords

Paeonia lactiflora Pall. Flowers Antioxidative Anti-inflammatory Free radicals Flavonoid
Jin, Y.-S., Chen, J., Jin, Y., Li, C., Chen, M., & Tao, J. (2016). Antioxidant and Anti-Inflammatory Activities of Ethyl Ether Extract from Paeonia lactiflora Pall. Flowers. Asian Journal of Chemistry, 28(5), 1144–1148. https://doi.org/10.14233/ajchem.2016.19615
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