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Electrochemical Determination of b-Blocker Alprenolol Hydrochloride in Its Pharmaceutical Formulations
Corresponding Author(s) : Nawal A. Alarfaj
Asian Journal of Chemistry,
Vol. 25 No. 11 (2013): Vol 25 Issue 11
Abstract
The construction and performance characteristics of alprenolol selective electrodes were developed. Two types of electrodes i.e., plastic membrane (I) and coated wire (II) were constructed based on the incorporation of alprenolol hydrochloride with phosphomolybdic acid. The influence of membrane composition, kind of plasticizer, pH of the test solution, soaking time and foreign ions on the electrodes were investigated. The electrodes showed a Nernstian response with a mean calibration graph slope of 58.54 ± 0.2 and 57.83 ± 0.4 mV/decade at 25 ºC for electrodes (I) and (II) respectively, over alprenolol hydrochloride concentration range from 3.0 × 10-6 - 1.0 × 10-2 and 5.0 × 10-6 - 1.0 × 10-2 mol/L, with detection limit of 1.6 × 10-6 mol/L and 2.5 × 10-6 mol/L for electrodes (I) and (II) respectively. The constructed electrodes gave average selective precision and accuracy within the pH range (3-8). Interferences from common cations, alkaloids, sugars, amino acids and drug excipients were reported. The results obtained by the proposed electrodes were also applied successfully to the determination of the drug in pharmaceutical preparations.
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References
J.V. Jackson, M.S. Moss and B. Widdop, Clark's Isolation and Identification of Drugs, edn. 2, p. 331 (1986).
V. Ranta, E. Toropainen,A. Talvitie, A. Auriola and A. Urtti, J. Chromatogr. B, 772, 81 (2002).
M.I. Maguregui, R.M. Alonso and R.M. Jiménez, J. Chromatogr. B, 674, 85 (1995).
J. Hermansson and C. von Bahr, J. Chromatogr., 227, 113 (1982).
C. Brunelli, C. Bicchi, A. Di Stilo, A. Salomone and M. Vincenti, J. Sep. Sci., 29, 2765 (2006).
H. Sirén, M. Saarinen, S. Hainari, P. Lukkari and M.-L. Riekkola, J. Chromatogr. A, 632, 215 (1993).
T. Yamaguchi, Y. Morimoto, Y. Sekine and M. Hashimoto, J. Chromatogr. A, 239, 609 (1982).
L. Gagyi, Á. Gyéresi and F. Kilár, J. Biochem. Biophys. Methods, 70, 1268 (2008).
A. Jouyban, M. Khoubnasabjafari, S. Yeghanli, S.C. Grosse and B.J. Clark, IL Farmaco, 58, 1039 (2003).
H.Y. Aboul-Enein and X.S. Xian, Analusis, 28, 855 (2000).
European Pharmacopoeia, Electronic edition (2011).
Information Bull. IUPAC, No. 43, Recommendations for Nomenclature of Ion-selective Electrodes (1975).
E. Linder, K. Toth and E. Pungor, Dynamic Characteristics of Ion-Selective Electrodes, Chemical Rubber Company (CRC) Press, Boca Raton, FL (1988).