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Vol. 25 No. 11 (2013): Vol 25 Issue 11

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Analysis of Interfering Substances in the Measurement of Malondialdehyde Content in Plant Leaves

https://doi.org/10.14233/ajchem.2013.14411
Yun Sheng Wang
Key Laboratory of Tea Biochemistry and Biotechnology, Ministry of Education in China, Anhui Agricultural University, Hefei, Anhui Province, P.R. China ; School of Life Sciences, Anhui Agricultural University, Hefei, Anhui Province, P.R. China
Mao Di Ding
School of Life Sciences, Anhui Agricultural University, Hefei, Anhui Province, P.R. China
Yunli Pang
Department of Mathematics, Xianyang Normal University, Xianyang, Shaanxi Province, P.R. China
Xun Gang Gu
Key Laboratory of Tea Biochemistry and Biotechnology, Ministry of Education in China, Anhui Agricultural University, Hefei, Anhui Province, P.R. China
Li Ping Gao
School of Life Sciences, Anhui Agricultural University, Hefei, Anhui Province, P.R. China
Tao Xia
Key Laboratory of Tea Biochemistry and Biotechnology, Ministry of Education in China,Anhui Agricultural University, Hefei,Anhui Province, P.R. China

Corresponding Author(s) : Li Ping Gao

gaolp62@126.com

Asian Journal of Chemistry, Vol. 25 No. 11 (2013): Vol 25 Issue 11

Abstract

The thiobarbituric acid reactive substances assay is an easy and quick assay for the assessment in plants of lipid peroxidation, in which malondialdehyde is derivatized. To analyze the applicability of this method, the malondialdehyde concentrations in the leaves of different plants were measured by the thiobarbituric acid method. For further separation of the interfering substances, fractional extraction and gas chromatography-mass spectrometry were used in this research. The results suggest that the lipid peroxidation is accurately measured by the thiobarbituric acid method with trichloroacetic acid as the extraction solution in most plants. However, the method was not suitable for measuring the malondialdehyde concentration in golden privet (Ligustrum × vicaryi) leaves. Negative malondialdehyde concentrations were obtained in golden privet leaves by this method.

Keywords

Gas chromatography-mass spectrometry Interfering substance Lipid peroxidation Malondialdehyde Thiobarbituric acid
Sheng Wang, Y., Di Ding, M., Pang, Y., Gang Gu, X., Ping Gao, L., & Xia, T. (2013). Analysis of Interfering Substances in the Measurement of Malondialdehyde Content in Plant Leaves. Asian Journal of Chemistry, 25(11), 6293–6297. https://doi.org/10.14233/ajchem.2013.14411
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