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Vol. 25 No. 11 (2013): Vol 25 Issue 11

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Isolation and Purification of Two Triterpenoids from the Chinese Medicinal Plant Fomes officinalis ames

https://doi.org/10.14233/ajchem.2013.14285
Huifeng Zhang
College of Life science, Jilin University, Changchun 130013, P.R. China ; Pharmaceutical analysis Department, Jilin Medical College, Jilin 132013, P.R. China
Bo Feng
College of Life science, Jilin University, Changchun 130013, P.R. China ; Pharmaceutical analysis Department, Jilin Medical College, Jilin 132013, P.R. China
Ke Liu
College of Life science, Jilin University, Changchun 130013, P.R. China
Shuying Guo
Pharmaceutical analysis Department, Jilin Medical College, Jilin 132013, P.R. China

Corresponding Author(s) : Shuying Guo

shuyingguocn@yeah.net

Asian Journal of Chemistry, Vol. 25 No. 11 (2013): Vol 25 Issue 11

Abstract

A high-speed counter-current chromatography method is developed for the separation and purification of two triterpenoids from the Chinese Uyghur medicinal plant Fomes officinalis ames. Crude triterpenoids were obtained from Fomes officinalis ames through ethanol extraction. Preparative high-speed counter-current chromatography with two-phase solvent systems made up of n-hexane-ethylacetate-methanol-water with a volume ratio of 1:1.5:1.2:1 (v/v) was successfully performed in a stepwise elution. The process yielded two relatively pure triterpenoids in a single run from 500 mg crude extract. The purities of the dehydrosulphurenic acid and 3-keto-dehydrosulphurenic acid were 96.8 % and 92.3 %, respectively.

Keywords

Fomes officinalis ames Counter-current chromatography Triterpenoids Preparative chromatography
Zhang, H., Feng, B., Liu, K., & Guo, S. (2013). Isolation and Purification of Two Triterpenoids from the Chinese Medicinal Plant Fomes officinalis ames. Asian Journal of Chemistry, 25(11), 6130–6132. https://doi.org/10.14233/ajchem.2013.14285
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