Copyright (c) 2013 AJC
This work is licensed under a Creative Commons Attribution 4.0 International License.
Influence of Auxins on Glucosinolate Biosynthesis in Hairy Root Cultures of Broccoli (Brassica oleracea var. italica)
Corresponding Author(s) : Sang Un Park
Asian Journal of Chemistry,
Vol. 25 No. 11 (2013): Vol 25 Issue 11
Abstract
One of the most significant health benefits of cruciferous vegetables results from the presence of biologically active compounds, glucosinolates. Broccoli (Brassica oleracea var. italica) is a nutritionally important crop grown all over the world. The primary purpose of this study was to evaluate the effect of auxins on glucosinolate biosynthesis in the hairy root of broccoli. Eight different glucosinolates, including glucoraphanin, gluconapin, 4-hydroxyglucobrassicin, glucoerucin, glucobrassicin, 4-methoxyglucobrassicin, gluconasturtiin and neoglucobrassicin, were identified by analysis of broccoli hairy root cultures. Low concentration of auxins (0.1 mg/ L) resulted in the highest accumulation of glucosinolates. Treatment with higher concentrations resulted less accumulation of glucosinolates than treatment with 0.1 mg/L of auxins. Among the tested compounds, treatment with 1 mg/L NAA resulted in the lowest accumulation of glucosinolates. Treatment with 0.1 mg/L IAA resulted in the accumulation of 2.2 times more glucosinolates than treatment with 1 mg/L NAA and 1.6 times more glucosinolates than was found in control. Treatment with 0.1 mg/L IBA resulted in the second highest accumulation of glucosinolates. Our results suggest that auxin treatment, especially IAA at a concentration of 0.1 mg/L could be used to mass produce glucosinolates from hairy root culture of broccoli.
Keywords
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX
- J.W. Fahey, Y. Zhang and P. Talalay, Proc. Nat. Acad Sci USA, 94, 10367 (1997).
- E.A.S. Rosa and A.S. Rodrigues, Hort. Sci., 36, 56 (2001).
- A. Podsedek, Food Sci. Technol., 40, 1 (2007).
- A.C. Kurilich, G.J. Tsau,A. Brown, L. Howard, B.P. Klein, E.H. Jeffery, M. Kushad, M.A. Wallig and J.A. Juvik, J. Agric. Food Chem., 47, 1576 (1999).
- J. Nilsson, K. Olsson, G. Engqvist, J. Ekvall, M. Olsson, M. Nyman and B. kesson, J. Sci. Food Agric., 86, 528 (2006).
- C.W.W. Beecher, Am. J. Clin. Nutr., 59, 1166 (1994).
- A. Agudo, L. Cabrera, P. Amiano, E. Ardanaz,A. Barricarte, T. Berenguer, M.D. Chirlaque, M. Dorronsoro, P. Jakszyn, N. Larranaga, C. Martinez, C. Navarro, J.R. Quiros, M.J. Sanchez, M.J. Tormo and C.A. Gonzalez, Am. J. Clin. Nutr., 85, 1634 (2007).
- P. Kroon and G. Williamson, J. Sci. Food Agric., 85, 1239 (2005).
- M.C. Christey and R.H. Braun RH. Methods, Mol. Biol., 286, 47 (2005).
- M.I. Georgiev, A.I. Pavlov and T. Bley, Appl. Microbiol. Biotechnol., 74, 1175 (2007).
- S. Srivastava and A.K. Srivastava, Crit. Rev. Biotechnol., 27, 29 (2007).
- F. Bourgaud, A. Gravot, S. Milesi and E. Gontier, Plant Sci., 161, 839 (2001).
- T. Murashige and F. Skoog, Physiol. Plantarum, 15, 473 (1962).
- S.J. Kim, C. Kawaharada, S. Jin, M. Hashimoto, G. Ishii and H. Yamauchi, Biosci. Biotechnol. Biochem., 71, 114 (2007).
- International Organization of Standardization, ISO 9167-1:1992(E), Geneva, Switzerland (1992).
- M. Sauerwein, T. Yamazaki and K. Shimomura, Plant Cell Rep., 9, 579 (1991).
- I. Bálványos, L. Kursinszki and E. Szõke, Plant Growth Regul., 34, 339 (2001).
- D. Washida, K. Shimomura, M. Takido and S. Kitanaka, Biol. Pharm. Bull., 27, 657 (2004).
- Y.S. Kim, X. Li, W.T. Park, M.R. Uddin, N.I. Park, Y.B. Kim, M.Y. Lee and S.U. Park, Plant Omics J., 5, 24 (2012
References
J.W. Fahey, Y. Zhang and P. Talalay, Proc. Nat. Acad Sci USA, 94, 10367 (1997).
E.A.S. Rosa and A.S. Rodrigues, Hort. Sci., 36, 56 (2001).
A. Podsedek, Food Sci. Technol., 40, 1 (2007).
A.C. Kurilich, G.J. Tsau,A. Brown, L. Howard, B.P. Klein, E.H. Jeffery, M. Kushad, M.A. Wallig and J.A. Juvik, J. Agric. Food Chem., 47, 1576 (1999).
J. Nilsson, K. Olsson, G. Engqvist, J. Ekvall, M. Olsson, M. Nyman and B. kesson, J. Sci. Food Agric., 86, 528 (2006).
C.W.W. Beecher, Am. J. Clin. Nutr., 59, 1166 (1994).
A. Agudo, L. Cabrera, P. Amiano, E. Ardanaz,A. Barricarte, T. Berenguer, M.D. Chirlaque, M. Dorronsoro, P. Jakszyn, N. Larranaga, C. Martinez, C. Navarro, J.R. Quiros, M.J. Sanchez, M.J. Tormo and C.A. Gonzalez, Am. J. Clin. Nutr., 85, 1634 (2007).
P. Kroon and G. Williamson, J. Sci. Food Agric., 85, 1239 (2005).
M.C. Christey and R.H. Braun RH. Methods, Mol. Biol., 286, 47 (2005).
M.I. Georgiev, A.I. Pavlov and T. Bley, Appl. Microbiol. Biotechnol., 74, 1175 (2007).
S. Srivastava and A.K. Srivastava, Crit. Rev. Biotechnol., 27, 29 (2007).
F. Bourgaud, A. Gravot, S. Milesi and E. Gontier, Plant Sci., 161, 839 (2001).
T. Murashige and F. Skoog, Physiol. Plantarum, 15, 473 (1962).
S.J. Kim, C. Kawaharada, S. Jin, M. Hashimoto, G. Ishii and H. Yamauchi, Biosci. Biotechnol. Biochem., 71, 114 (2007).
International Organization of Standardization, ISO 9167-1:1992(E), Geneva, Switzerland (1992).
M. Sauerwein, T. Yamazaki and K. Shimomura, Plant Cell Rep., 9, 579 (1991).
I. Bálványos, L. Kursinszki and E. Szõke, Plant Growth Regul., 34, 339 (2001).
D. Washida, K. Shimomura, M. Takido and S. Kitanaka, Biol. Pharm. Bull., 27, 657 (2004).
Y.S. Kim, X. Li, W.T. Park, M.R. Uddin, N.I. Park, Y.B. Kim, M.Y. Lee and S.U. Park, Plant Omics J., 5, 24 (2012