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Spectrophotometric Determination of Protein with DBC-Chlorophosphonazo
Corresponding Author(s) : Qing-Zhou Zhai
Asian Journal of Chemistry,
Vol. 25 No. 10 (2013): Vol 25 Issue 10
Abstract
A new spectrophotometric method for the determination of protein has been developed using the property that bovine serum albumin and DBC-chlorophosphonazo form a compound. The maximum absorption wavelength of the compound is 606 nm. Beer's law is obeyed over the range of 25-250 μg/mL of bovine serum albumin contents. The detection limit of this method is 9.52 μg/mL. Protein content in human serum has been successfully determined.
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- Q.Z. Zhai, X.X. Zhang and X.T. Yang, J. Changchun Uni. Sci. Technol. (Nat. Sci. Ed.), 32, 165 (2009).
- J.S. Wang, B.S. Liu, Y.M. Sun, J. Bai and C.R. Zhao, Chin. J. Spectrosc. Lab., 22, 599 (2005).
- M.L. Guo and Y.M. Jiang, Prog. Biochem. Biophys., 23, 558 (1996).
- M.M.A. Bradford, Anal. Biochem., 72, 248 (1976).
- H.L. Chen, S.P. Liu and Z.F. Liu, Chin. J. Anal. Chem., 32, 698 (2004).
- J.M. Li, X.X. Zhang, L.L. Fan, P. Dong and Q.Z. Zhai, Chem. Res. Appl., 17, 686 (2005).
- G.Q. Zhan, W.X. Yu, C.T. Wei and J.J. Lu, J. Anal. Sci., 22, 309 (2006).
- J.M. Pan, J.Z. Li, Q.Y. Zhang and G.Z. Fang, New Chromogenic Reagents and Their Application in Spectrophotometry. Beijing: Chemical Industry Press, China, p. 34 (2003).
References
Q.Z. Zhai, X.X. Zhang and X.T. Yang, J. Changchun Uni. Sci. Technol. (Nat. Sci. Ed.), 32, 165 (2009).
J.S. Wang, B.S. Liu, Y.M. Sun, J. Bai and C.R. Zhao, Chin. J. Spectrosc. Lab., 22, 599 (2005).
M.L. Guo and Y.M. Jiang, Prog. Biochem. Biophys., 23, 558 (1996).
M.M.A. Bradford, Anal. Biochem., 72, 248 (1976).
H.L. Chen, S.P. Liu and Z.F. Liu, Chin. J. Anal. Chem., 32, 698 (2004).
J.M. Li, X.X. Zhang, L.L. Fan, P. Dong and Q.Z. Zhai, Chem. Res. Appl., 17, 686 (2005).
G.Q. Zhan, W.X. Yu, C.T. Wei and J.J. Lu, J. Anal. Sci., 22, 309 (2006).
J.M. Pan, J.Z. Li, Q.Y. Zhang and G.Z. Fang, New Chromogenic Reagents and Their Application in Spectrophotometry. Beijing: Chemical Industry Press, China, p. 34 (2003).