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Vol. 25 No. 8 (2013): Vol 25 Issue 8

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A New Method of Discrimination for Peaks of Enantiopure Compounds in Chiral Separation of HPLC

https://doi.org/10.14233/ajchem.2013.13975
Zhi-Jian He
College of Chemistry and Chemical Engineering, Mianyang Normal University, Mianyang, P.R. China ; Department of Pharmaceutical and Biological Engineering, Sichuan University, No. 24 South Section 1, Yihuan Road, Chengdu 610065, P.R. China
Yi-Wen Zhang
Department of Pharmaceutical and Biological Engineering, Sichuan University, No. 24 South Section 1, Yihuan Road, Chengdu 610065, P.R. China
Shun Yao
Department of Pharmaceutical and Biological Engineering, Sichuan University, No. 24 South Section 1, Yihuan Road, Chengdu 610065, P.R. China
Bin Tu
Department of Pharmaceutical and Biological Engineering, Sichuan University, No. 24 South Section 1, Yihuan Road, Chengdu 610065, P.R. China
Hang Song
Department of Pharmaceutical and Biological Engineering, Sichuan University, No. 24 South Section 1, Yihuan Road, Chengdu 610065, P.R. China

Corresponding Author(s) : Hang Song

hangsong@scu.edu.cn; 376971478@qq.com

Asian Journal of Chemistry, Vol. 25 No. 8 (2013): Vol 25 Issue 8

Abstract

Diode array detector is one of the most popular detectors of high performance liquid chromatography (HPLC). With the full-band scanning, it has been used extensively for the qualitative and quantitative analysis of components. In this paper, a few groups of enantiomers, diastereomers, isomers and homologue were chosen as analytes and a new method of discrimination for their peaks in chromatogram of HPLC was tried to be developed. By using diode array detector, we obtained peak characteristics for them. By comparing chromatograms of enantiomers, diastereomers, isomers and homologue, we find that only the chromatograms of the enantiomers and diastereomers have considerable consistency. Then a new method of discrimination for peaks of enantiopure compounds by diode array detector has been established. This method can be used for research of chiral separation.

Keywords

High performance liquid chromatography Diode array detector Chiral separation Enantiomer Diastereomer Isomer
He, Z.-J., Zhang, Y.-W., Yao, S., Tu, B., & Song, H. (2013). A New Method of Discrimination for Peaks of Enantiopure Compounds in Chiral Separation of HPLC. Asian Journal of Chemistry, 25(8), 4351–4355. https://doi.org/10.14233/ajchem.2013.13975
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